Ikhithi ye-PCR ye-Plant Leaf yase-China (ngaphandle Kwamathuluzi Esampula) Iphrothokholi Eqondile ye-PCR evela kumkhiqizi wezinto zezitshalo kanye Nomphakeli |I-Foregene

Ikhithi ye-PCR eqondile yeqabunga (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material

Ikhithi ye-PCR eqondile yeqabunga lesitshalo (ngaphandle kwamathuluzi okusampula) Iphrothokholi ye-PCR eqondile evela ku-Plant Material Yesithombe Esifakiwe

Incazelo yekhithi:

Sebenzisa ngokuqondile iqabunga noma i-lysate yeqabunga njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.

◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)

◮Impahla encane

◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya

◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela

◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50

◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo

Lo mkhiqizo usebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze uhlukanise amaqabunga ezitshalo.I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanza.Ngemva kokwengeza ama-primer, i-PCR Mix yale khithi ingasetshenziselwa ukukhulisa.

Imininingwane

0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	Isilondolozi se-P1
	Isilondolozi se-P2
	6×I-DNA Loading Buffer
Ingxenye II	2× Leaf PCR Easy^TMHlanganisa

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well

Imingcele yekhithi

Isicelo: ukuhlonza okushintshwe izakhi, i-genotyping, njll.
Isampula: iqabunga lesitshalo
Isikali: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)
Ibanga lokutholwa: isiqeshana esiqondiwe ≤1kb