Tshala iqabunga lekhithi le-PCR eliqondile

Tshala iqabunga lekhithi le-PCR eliqondile

Incazelo Yekhithi:

Lo mkhiqizo usebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukuze uvuse amaqabunga ezitshalo. I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanzwa. Ama-Primers angangezwa futhi i-PCR Mix yale kit ingasetshenziselwa ukukhulisa.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Imininingwane

0x 20 ×l rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Izingxenye Kit

Ingxenye I

I-Buffer P1

Ibhafa P2

6×I-DNA Loading Buffer

Ingxenye II

2× Leaf PCR KululaTM Hlanganisa

Izici nezinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi futhi okubizayo
■ Izinto ezingaphansi
■ Isampula esilula singabhekana nokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Hlanganisa, ukunciphisa amaphutha wokulayisha amasampula nesikhathi sokulungiselela uhlelo
■ Ukulungiselela ama-template okusheshayo kungaqedwa ngemizuzu eyi-10, ukuphendula kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngo-96-well PCR plate

Amapharamitha ekhithi

Isicelo: ukukhonjwa kofuzo, genotyping, njll.
Isampula: tshala iqabunga
Isilinganiso: Ububanzi 2-3mm (indlela eqondile), ubukhulu 5-7mm (indlela ye-lysis)
Ibanga lokuthola: isiqeshana sethagethi ≤1kb

Ukuhamba komsebenzi

Plant Leaf Direct PCR Kit04

I-Electropherogram

Plant Leaf Direct PCR Kit05

  • Langaphambilini
  • Olandelayo:

  • Bhala umyalezo wakho lapha bese uwuthumela kithi