I-China Plant Seed (enkulu nemaphakathi) I-PCR eqondile Ikhithi II-UNG(ngaphandle Kwamathuluzi Esampula) Umkhiqizi Nomhlinzeki |I-Foregene

Imbewu Yezitshalo (enkulu nephakathi nendawo) I-PCR Plus Kit II-UNG eqondile (ngaphandle kwamathuluzi okusampula)

Imbewu Yezitshalo (enkulu nephakathi nendawo) I-Direct PCR Plus Kit II-UNG(ngaphandle Kwamathuluzi Okusampula) Isithombe Esifakiwe

Incazelo yekhithi:

Sebenzisa ngokuqondile i-polysaccharide polyphenol seed lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.

◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.

◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo

Lo mkhiqizo usebenzisa isistimu yokusabela ye-lysis eyingqayizivele, engakhulula ngokushesha i-DNA ye-genomic kumasampula embewu yezitshalo ane-polysaccharides ephezulu nama-polyphenols (njengobhanana, ukotini, njll.) ukuze isetshenziswe ekuphenduleni kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Ukuze kuhlangatshezwane nezidingo zokuhlolwa kokutholwa okuhlukile, le kit ingasebenzisa izinhlobo eziningi zamasampuli (njengembewu ephelele, ukusikeka kwezicubu ezincane, noma amasampula aphansi embewu eminingi) ukuze kuhlolwe.Phakathi kwazo, amasampula embewu asadinga ukuhluma, ungakhetha ukusika izingcezu zezicubu ezincane (1-5mg) ngaphandle kwemibungu yembewu ukuze uhlole;ngokuhlolwa okudinga amasampula nokuhlolwa enanini elikhulu lamasampuli, ungakhetha ukuhlanganisa amasampuli amaningi futhi uwahlanganise.Ngemva kokugaya zibe izinhlayiya ezinobubanzi obungaba ngu-0.5mm, ukuhlolwa kungenziwa (okungenani u-0.1% wesampula okuhlosiwe kungatholwa).

Imininingwane

50T,200T,500T,2000T

Izingxenye zekhithi

Plant Seed Direct PCR Plus Kit II-UNG Imbewu yezitshalo ye-Polysaccharide polyphenol (enkulu naphakathi) eqondile ye-PCR kit-UNG
Ukwakheka kwekhithi		I-TP-0318T	I-TP-03181	I-TP-03182	TP-03183
Ukwakheka kwekhithi		50 T	200 T	500 T	2000 T
Ingxenye I	Isivimbeli SSP1	24ml	96ml	120ml×2	480ml×2
	Isilondolozi se-SP2	3ml	10ml	25ml	100ml
	I-6× I-DNA Yokulayisha Ibhafa	1.5ml	1.5ml	1.5ml	1.5ml×4
Ingxenye II	Isivimbeli SSP2	8ml	32ml	80ml	80mlx4
Ingxenye II	2× Imbewu PCR EasyTM Mix	500μl	1mlx2	1.7ml×3	1.7ml×12
Imanuwali		1	1	1	1

Izici nezinzuzo

- Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo.

- Isidingo sesampula sincane, thatha nje imbewu eyodwa.

- Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukusebenza kulula.

- Uhlelo lwe-PCR olulungiselelwe lwenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.

- Isistimu ye-PCR yokulwa nokungcoliswa 2× Imbewu PCR Kulula^TM I-Mix (UNG), engaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukucaciswa nokunemba kokukhulisa.