Isikhathi Sangempela PCR Easyᵀᴹ-SYBR Green I kit
Incazelo yekhithi:
I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza
I-Specific-optimized buffer kanye ne-hot-start Taq enzyme ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer
Ukuzwela okuphezulu-kungathola amakhophi aphansi esifanekiso
Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR
Izincazelo zekhithi
I-2×Real PCR EasyTMI-Mix-SYBR ihlinzekwa yi-Real Time PCR EasyTM-Ikhithi ye-SYBR Green I iwuhlelo olusha lwe-premix olusebenzisa i-SYBR Green I ye-Real Time PCR ukusabela kokukhulisa, okungathuthukisa kakhulu ukucaciswa komkhiqizo nokuzwela kokusabela .I-ROX inikezwa njengodayi wokulawula wangaphakathi.Amandla e-fluorescence ale khithi aphindwe izikhathi ezingu-3-5 kunemikhiqizo efanayo, engazwela kakhulu futhi ibonise ngokunembile ukugxila kwesifanekiso se-DNA esihlosiwe.
2×I-PCR Yangempela KululaTMI-Mix-SYBR iqukethe i-Taq DNA Polymerase eyingqayizivele ye-Foregene.Uma iqhathaniswa nama-enzyme e-Taq ajwayelekile, inezinzuzo zokusebenza kahle kokukhulisa i-amplification, ikhono elithile eliqinile lokukhulisa kanye nezinga lokungafani eliphansi.Inganciphisa ukukhulisa okungaqondile futhi ithuthukise ukunemba kwe-PCR.
Imininingwane
| Isikhathi Sangempela PCR Easyᵀᴹ-SYBR Green I Kit | ||||
| Ukwakheka kwekhithi(20μl system) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| I-2 × I-PCR yangempela kululaTMI-Mix-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| I-50 × I-ROX Reference Dye | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| I-DNase-Free ddH2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Isiyalezo | 1 | 1 | 1 | 1 |
Izici&izinzuzo
■ I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza
■ Ibhafa ecacisiwe—elungiselelwe kahle kanye ne-enzyme ye-Taq eqala ukushisa ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer.
■ Ukuzwela okuphezulu—kukwazi ukubona amakhophi aphansi esifanekiso
■ Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR
Isicelo sekhithi
Ukuhlaziywa kwe-qPCR
Ukugeleza komsebenzi
Umfanekiso
Isitoreji nempilo yeshelufu
Le kit kufanele igcinwe kude nokukhanya futhi kufanele igcinwe ku -20 ℃.Uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ isikhathi esifushane (izinsuku eziyi-10).
