Abasebenzi bethu ngokuqeqeshwa abanolwazi.Ulwazi olunekhono, umuzwa oqinile womhlinzeki, ukwanelisa ukusekela okufunwa abathengi be-SuperLowest Price Dnasecure Plant Kit ye-DNA Extraction Nucleic Acid Purification, Sifisa ukukhetha leli thuba ukuze sithole ubudlelwano besikhathi eside bebhizinisi elincane namakhasimende avela kuwo wonke umhlaba.
Abasebenzi bethu ngokuqeqeshwa abanolwazi.Ulwazi olunamakhono anekhono, umuzwa oqinile womhlinzeki, ukwanelisa izidingo zosekelo zabathengiIkhithi ye-Reagent ye-Nucleic Acid yase-China kanye nekhithi ye-Reagent ye-Nucleic Acid Extraction, Imikhiqizo yethu yaziwa kabanzi futhi ithenjwa ngabasebenzisi futhi ingahlangabezana ngokuqhubekayo nezidingo zezomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze athole ubudlelwano bebhizinisi besikhathi esizayo futhi azuze impumelelo efanayo!

Izincazelo zekhithi

Le kit yamukela i-Foregene Protease Plus entsha kanye ne-BL1 kanye ne-BL2 buffer systems ehlukile, engagaya ngokuphelele amasampula egazi e-anticoagulant ngesikhathi esifushane, ngaleyo ndlela igweme ukuwohloka kwe-DNA kuze kufike ezingeni eliphezulu futhi ithole inani eliphezulu le-DNA ye-genomic.Uhlelo olusheshayo lokucutshungulwa kwegazi kanye nokusebenza okulula kwekholomu ye-DNA-Kuphela kwenza kube lula kakhulu ukukhishwa kwe-genome yegazi, okwenza i-DNA ye-genomic ingatholakala yekhwalithi ephezulu kanye nokuhlanzeka okuphezulu phakathi nemizuzu engu-40.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu ye-centrifuge yale kit iwuhlobo olusha lwezinto ezibonakalayo ezihlukile enkampanini yethu, ezingabopha i-DNA ngendlela efanele futhi ngokuqondile, futhi ingasusa i-RNA, ukungcola, amaprotheni, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngokwezinga eliphezulu.Izingcezu ze-DNA ezitholiwe zinkulu, zihlanzekile, zizinzile futhi zithembekile.Umthamo omkhulu wokuthwala wekholomu ye-DNA-Only ngu-80 μg we-DNA.I-DNA etholiwe ingasetshenziswa ekuhlolweni kwebhayoloji yamangqamuzana njengokugaya kwe-enzyme, i-PCR, i-Southern hybridization kanye nokwakhiwa komtapo wolwazi.

Ikhithi ingacubungula kuze kufike ku-1ml wegazi ngesikhathi ukuze ithole inani elanele le-DNA ye-genomic ephezulu.

Imininingwane

*: I-Buffer BL2 kanye ne-Buffer PW iqukethe usawoti owuketshezi ocasulayo.Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.

-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, i-4°C centrifugation noma i-ethanol precipitation ye-DNA ayidingeki.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent esetshenziswayo.

-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.

Ukugeleza komsebenzi

Isitoreji nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

Isixazululo se-Foregene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);izosebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.

Abasebenzi bethu ngokuqeqeshwa abanolwazi.Ulwazi olunekhono, umuzwa oqinile womhlinzeki, ukwanelisa ukusekela okufunwa abathengi be-SuperLowest Price Dnasecure Plant Kit ye-DNA Extraction Nucleic Acid Purification, Sifisa ukukhetha leli thuba ukuze sithole ubudlelwano besikhathi eside bebhizinisi elincane namakhasimende avela kuwo wonke umhlaba.
Inani Eliphansi KakhuluIkhithi ye-Reagent ye-Nucleic Acid yase-China kanye nekhithi ye-Reagent ye-Nucleic Acid Extraction, Imikhiqizo yethu yaziwa kabanzi futhi ithenjwa ngabasebenzisi futhi ingahlangabezana ngokuqhubekayo nezidingo zezomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze athole ubudlelwano bebhizinisi besikhathi esizayo futhi azuze impumelelo efanayo!