I-Viral DNA&RNA Isolation Kit Viral DNA kanye ne-RNA Extraction Purification Purification Kits
Imininingwane
50 Preps, 200 Preps
I-Viral RNA Nucleic Acid Purification Extraction Isolation Kit isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA yegciwane lezinga eliphezulu kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olunganaseli, kanye ne-cell culture supernatant.Ikhithi yengeza ngokuqondile i-Linear Acrylamide, engathwebula kalula amanani amancane e-RNA kumasampuli.Ikholomu Ye-RNA Kuphela ingabopha i-RNA ngokuphumelelayo.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.
Yonke ikhithi ayinayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe.I-Buffer viRW1 kanye ne-Buffer viRW2 ingaqinisekisa ukuthi i-viral nucleic acid etholwe ayinawo amaprotheni, i-nuclease noma okunye ukungcola, engasetshenziswa ngokuqondile ekuhloleni kwebhayoloji yamangqamuzana angezansi.
Izingxenye zekhithi
| Umugqa we-Acrylamide |
| Isilondolozi se-DRL |
| Isilondolozi RW1, Isilondolozi RW2 |
| I-ddH yamahhala ye-RNase2O |
| Ikholomu ye-DNA/RNA |
| Iziyalezo |
Izici&izinzuzo
■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Isivuno esiphezulu se-nucleic acid: Ikholomu ye-DNA/RNA kuphela kanye nefomula eyingqayizivele ingahlanza ngokuphumelelayo i-DNA ne-RNA.
■ Umthamo omkhulu wokucubungula isampula: amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-20.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.
Isicelo sekhithi
Ilungele ukukhishwa nokuhlanzwa kwe-viral nucleic acid kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olungenamaseli kanye ne-cell culture supernatant.
Ukugeleza komsebenzi
Umdwebo
Isitoreji kanye Nempilo yeshelufu
■ Le kit ingagcinwa izinyanga ezingu-24 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2–8℃.
■ Isixazululo se-Linear Acrylamide singagcinwa ekamelweni lokushisa izinsuku ezingu-7;ngemva kokuthola ikhithi, sicela uyikhiphe futhi uyigcine ku-20°C.
■ Ngemva kokwengeza i-Linear Acrylamide ku-Buffer DRL, ingagcinwa ku-2-8°C kufika ku-48h.Sicela usebenzise isixazululo esenziwe ngomumo.
Umhlahlandlela Wokuhlaziya Inkinga
Okulandelayo ukuhlaziya izinkinga ezingase kuhlangatshezwane nazo ekukhishweni kwe-viral DNA/RNA, ngethemba lokuba usizo ekuhloleni kwakho.Ngaphezu kwalokho, kwezinye izinkinga zokuhlola noma zobuchwepheshe ngaphandle kwemiyalo yokusebenza nokuhlaziya inkinga, sinikele ngosekelo lobuchwepheshe ukuze sikusize.Uma unezinye izidingo, sicela usithinte: 028-83360257 noma E-mail:
Tech@foregene.com.
Akukho ukukhishwa kwe-nucleic acid noma isivuno esiphansi se-nucleic acid
Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-nucleic acid, indlela yokusebenza, ivolumu ye-elution, njll.
Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Ukugeza kweqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation yenziwa ngesikhathi senqubo.
Ukusikisela: Sebenzisa ekamelweni lokushisa (15-25°C) kuyo yonke inqubo, ungabhavi eqhweni kanye nokushisa okuphansi kwe-centrifugation.
2. Isampula ligcinwe ngendlela engafanele noma isampula ligcinwe isikhathi eside kakhulu.
Okutuswayo: Gcina amasampula ku -80°C futhi ugweme ukuqhwa okuphindaphindiwe nokuncibilika;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-nucleic acid.
3. Isampula yesampula enganele.
Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesisombululo sokusebenza se-lysis kuxutshwe kahle futhi kufakwe endaweni yokushisa yegumbi (15-25°C) imizuzu eyi-10.
4. Ukwengezwa okungalungile kwe-eluent.
Isiphakamiso: Qiniseka ukuthi i-ddH2O Engenayo i-RNase yengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza, futhi ungayibeki eringini yekholomu yokuhlanza.
5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RW2.
Ukusikisela: Sicela ulandele imiyalelo, wengeze ivolumu efanele ye-ethanol ephelele ku-Buffer RW2 futhi uhlanganise kahle ngaphambi kokusebenzisa ikhithi.
6. Ivolumu yesampula engafanele.
Isiphakamiso: U-200µl wesampula ucutshungulwa kuwo wonke u-500µl we-Buffer DRL.Ukucutshungulwa kwesampula okweqile kuzoholela emvuzweni ephansi yokukhipha i-nucleic acid.
7. Ivolumu ye-elution engafanele noma ukungezwani okuphelele.
Isincomo: Ivolumu ecacile yekholomu yokuhlanza ingu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi unwebe isikhathi kuzinga lokushisa legumbi ngemva kokwengeza i-ddH2O eshisiwe ye-RNase-Free, njenge-5-10min.
8. I-Ethanol ihlala kukholamu ngemva kokugeza nge-Buffer RW2.
Isiphakamiso: Uma i-ethanol isala ngemva kokufakwa phakathi kwe-centrifugation ne-Buffer RW2 imizuzu emi-2, ikholomu ingabekwa ekamelweni lokushisa imizuzu emi-5 ngemva kwe-centrifugation ukuze ikhiphe ngokuphelele i-ethanol esele.
I-nucleic acid ehlanzekile iyancipha
Izinga le-nucleic acid ehlanzekile lihlobene nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, ukusebenza nezinye izici.Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Amasampula aqoqiwe awagcinwanga ngesikhathi.
Ukusikisela: Uma isampuli ingasetshenziswa ngesikhathi ngemva kokuqoqwa, sicela uyigcine ku -80°C ezingeni lokushisa eliphansi ngokushesha.Ukuze uthole i-RNA, zama ukusebenzisa amasampuli asanda kuqoqwa.
2. Qoqa amasampula bese uwaqandisa futhi uncibilike ngokuphindaphindiwe.
Ukusikisela: Gwema ukuqanda nokuncibilika (hhayi ngaphezu kokukodwa) ngesikhathi sokuqoqwa nokugcinwa kwamasampula, ngaphandle kwalokho isivuno se-nucleic acid sizoncipha.
3. I-RNase yethulwa egumbini lokuhlinza noma amagilavu alahlwayo, imaski, njll. awagqokwa.
Okutuswayo: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu ekamelweni lokusebenza elihlukile le-RNA, futhi itafula laselabhorethri kufanele lihlanzwe ngaphambi kokuhlolwa.
Gqoka amagilavu alahlwayo namamaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase ngezinga elikhulu kakhulu.
4. I-reagent yonakaliswe i-RNase ngesikhathi sokusetshenziswa.
Isincomo: Faka esikhundleni se-Viral DNA/RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.
5. Amashubhu e-centrifuge namathiphu e-piette asetshenziselwa ukukhwabanisa kwe-RNA angcoliswe yi-RNase.
Ukusikisela: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu e-pipette, amapayipi, njll. asetshenziselwa ukukhipha i-RNA konke Akuna-RNAse.
I-nucleic acid ehlanjululwe ithinta ukuhlolwa okungezansi komfula
I-DNA ne-RNA ehlanjululwe yikholomu yokuhlanza, uma i-ion kasawoti kanye nokuqukethwe kwamaprotheni kuphezulu kakhulu, kuzothinta ukuhlolwa okuya phansi komfula, okufana nalokhu: Ukukhulisa i-PCR, ukuloba okuhlanekezelwe, njll.
1. I-DNA ekhishiwe kanye ne-RNA inama-ion kasawoti asele.
Isiphakamiso: Qinisekisa ukuthi ivolumu elungile ye-ethanol ephelele yengezwa ku-Buffer RW2, futhi ugeze ikholomu yokuhlanza kabili ngesivinini sokuhlanganisa esishiwo emiyalweni yokusebenza;Yenza i-centrifugation ukuze unciphise ukungcoliswa kwe-ion kasawoti.
2. I-DNA ekhishiwe kanye ne-RNA inezinsalela ze-ethanol.
Ukusikisela: Ngemva kokuqinisekisa ukugeza nge-Buffer RW2, yenza i-tube centrifugation engenalutho ngesivinini se-centrifugation emiyalweni yokusebenza;uma kusekhona izinsalela ze-ethanol, ungakwazi ukufaka i-centrifuge ishubhu elingenalutho bese ulibeka endaweni yokushisa yasekamelweni imizuzu engu-5 ukuze ususe insalela ye-ethanol ngezinga elikhulu kakhulu.
Amamanuwali wemiyalo:
I-Viral DNA&RNA Isolation Kit Instruction Manual
