Ikhithi encane ye-DNA yegazi
Izincazelo zekhithi
Le kit yamukela i-Foregene Protease Plus entsha kanye ne-BL1 kanye ne-BL2 buffer systems ehlukile, engagaya ngokuphelele amasampula egazi e-anticoagulant ngesikhathi esifushane, ngaleyo ndlela igweme ukuwohloka kwe-DNA kuze kufike ezingeni eliphezulu futhi ithole inani eliphezulu le-DNA ye-genomic.Uhlelo olusheshayo lokucutshungulwa kwegazi kanye nokusebenza okulula kwekholomu ye-DNA-Kuphela kwenza kube lula kakhulu ukukhishwa kwe-genome yegazi, okwenza i-DNA ye-genomic ingatholakala yekhwalithi ephezulu kanye nokuhlanzeka okuphezulu phakathi nemizuzu engu-40.
Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu ye-centrifuge yale kit iwuhlobo olusha lwezinto ezibonakalayo ezihlukile enkampanini yethu, ezingabopha i-DNA ngendlela efanele futhi ngokuqondile, futhi ingasusa i-RNA, ukungcola, amaprotheni, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngokwezinga eliphezulu.Izingcezu ze-DNA ezitholiwe zinkulu, zihlanzekile, zizinzile futhi zithembekile.Umthamo omkhulu wokuthwala wekholomu ye-DNA-Only ngu-80 μg we-DNA.I-DNA etholiwe ingasetshenziswa ekuhlolweni kwebhayoloji yamangqamuzana njengokugaya kwe-enzyme, i-PCR, i-Southern hybridization kanye nokwakhiwa komtapo wolwazi.
Ikhithi ingacubungula kuze kufike ku-1ml wegazi ngesikhathi ukuze ithole inani elanele le-DNA ye-genomic ephezulu.
Imininingwane
Ikhithi encane ye-DNA yegazi | |||
Okuqukethwe kwekhithi | DE-05111 | DE-05112 | DE-05113 |
50 T | 100 T | 250 T | |
Isilondolozi BL1 | 15 ml | 30 ml | 75 ml |
Isigcinalwazi BL2 * | 15 ml | 30 ml | 75 ml |
I-Buffer DC | 100 ml | 200 ml | 500 ml |
Isilondolozi PW * | 25 ml | 50 ml | 125 ml |
Isilondolozi se-WB1 | 15 ml | 30 ml | 75 ml |
Isilondolozi EB | 10 ml | 20 ml | 50 ml |
I-Foregene Protease Plus | 1.25 ml | 2.5 ml | 6.5 ml |
Ikholomu Ye-DNA Kuphela | 50 | 100 | 250 |
Imanuwali | 1 | 1 | 1 |
*: I-Buffer BL2 kanye ne-Buffer PW iqukethe usawoti owuketshezi ocasulayo.Sicela ugqoke amagilavu futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.
Izici&izinzuzo
-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.
-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, i-4°C centrifugation noma i-ethanol precipitation ye-DNA ayidingeki.
-Ukuphepha: Akukho kukhishwa kwe-organic reagent esetshenziswayo.
-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.
Isicelo sekhithi
Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.
Ukugeleza komsebenzi
Isitoreji nempilo yeshelufu
Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
Isixazululo se-Foregene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);izosebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.