• facebook
  • linkedin
  • youtube
isibhengezo_sekhasi

Ikhithi encane ye-DNA yegazi

Incazelo yekhithi:

Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (<1ml).

Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

amandla angaphambili


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo zekhithi

Le kit yamukela i-Foregene Protease Plus entsha kanye ne-BL1 kanye ne-BL2 buffer systems ehlukile, engagaya ngokuphelele amasampula egazi e-anticoagulant ngesikhathi esifushane, ngaleyo ndlela igweme ukuwohloka kwe-DNA kuze kufike ezingeni eliphezulu futhi ithole inani eliphezulu le-DNA ye-genomic.Uhlelo olusheshayo lokucutshungulwa kwegazi kanye nokusebenza okulula kwekholomu ye-DNA-Kuphela kwenza kube lula kakhulu ukukhishwa kwe-genome yegazi, okwenza i-DNA ye-genomic ingatholakala yekhwalithi ephezulu kanye nokuhlanzeka okuphezulu phakathi nemizuzu engu-40.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu ye-centrifuge yale kit iwuhlobo olusha lwezinto ezibonakalayo ezihlukile enkampanini yethu, ezingabopha i-DNA ngendlela efanele futhi ngokuqondile, futhi ingasusa i-RNA, ukungcola, amaprotheni, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngokwezinga eliphezulu.Izingcezu ze-DNA ezitholiwe zinkulu, zihlanzekile, zizinzile futhi zithembekile.Umthamo omkhulu wokuthwala wekholomu ye-DNA-Only ngu-80 μg we-DNA.I-DNA etholiwe ingasetshenziswa ekuhlolweni kwebhayoloji yamangqamuzana njengokugaya kwe-enzyme, i-PCR, i-Southern hybridization kanye nokwakhiwa komtapo wolwazi.

Ikhithi ingacubungula kuze kufike ku-1ml wegazi ngesikhathi ukuze ithole inani elanele le-DNA ye-genomic ephezulu.

Imininingwane

Ikhithi encane ye-DNA yegazi

Okuqukethwe kwekhithi

DE-05111

DE-05112

DE-05113

50 T

100 T

250 T

Isilondolozi BL1

15 ml

30 ml

75 ml

Isigcinalwazi BL2 *

15 ml

30 ml

75 ml

I-Buffer DC

100 ml

200 ml

500 ml

Isilondolozi PW *

25 ml

50 ml

125 ml

Isilondolozi se-WB1

15 ml

30 ml

75 ml

Isilondolozi EB

10 ml

20 ml

50 ml

I-Foregene Protease Plus

1.25 ml

2.5 ml

6.5 ml

Ikholomu Ye-DNA Kuphela

50

100

250

Imanuwali

1

1

1

*: I-Buffer BL2 kanye ne-Buffer PW iqukethe usawoti owuketshezi ocasulayo.Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.

-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, i-4°C centrifugation noma i-ethanol precipitation ye-DNA ayidingeki.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent esetshenziswayo.

-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.

Ukugeleza komsebenzi

Ikhithi ye-Blood DNA Mini (<1ml)

Isitoreji nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

Isixazululo se-Foregene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);izosebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.


  • Okwedlule:
  • Olandelayo:

  • Bhala umyalezo wakho lapha futhi usithumelele wona

    OkuhlobeneImikhiqizo