Iqabunga lesitshalo Direct PCR kit-UNG (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material
Izincazelo
Ngokusekelwe kukhithi ye-Plant Leaf Direct PCR, i-dUTP isetshenziswa esikhundleni se-dTTP, futhi i-enzyme ye-UNG engehlisa isithunzi isifanekiso esiqukethe i-dUTP.Ngale ndlela, ukucaciswa nokunemba kokukhuliswa kuyaqinisekiswa, futhi inkinga yokungcoliswa komkhiqizo we-PCR okungenzeka ngesikhathi sokuhlolwa kofuzo okukhulu kuyavinjelwa.
Imininingwane
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Izingxenye zekhithi
Ingxenye I | Isilondolozi se-P1 |
Isilondolozi se-P2 | |
6×I-DNA Loading Buffer | |
Ingxenye II | 2× Leaf PCR EasyTMImiksi(UNG) |
Izici&izinzuzo
■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well
Imingcele yekhithi
Isicelo: ukuhlonza okushintshwe izakhi, i-genotyping, njll.
Isampula: iqabunga lesitshalo
Isikali: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)
Ibanga lokutholwa: isiqeshana esiqondiwe ≤1kb
Ukugeleza komsebenzi
Electropherogram
Amaviki amabili emiphumela yokutholwa kwe-PCR Mix ngaphansi kwesimo sokungcoliswa komkhiqizo wokukhulisa i-PCR.