I-Foregene DNA Identification System 30A (Engakhishiwe)
Incazelo yekhithi:
FOREGENE DNA identification system 30A isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuzekhulisa 29i-autosomalSIndawo ye-TR, eyodwaI-Amel locus kanye ne-Yindel locus eyodwa ngesikhathi esisodwa, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, i-FTAamakhadi, ama-swabs kakotini, amakhadi amathe,futhi mi-outh swab.
Incazelo
FOREGENE DNA identification system 30A isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuzekhulisa 29i-autosomalSIndawo ye-TR, eyodwaI-Amel locus kanye ne-Yindel locus eyodwa ngesikhathi esisodwa, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, i-FTAamakhadi, ama-swabs kakotini, amakhadi amathe,futhi mi-outh swab.
Okuqukethwe kwekhithi
Ukulungiselela Uhlelo Lokukhulisa
Ithebula 1: Ukwakheka okulungiselelwe kokusabela kokukhulisa Okujwayelekile (okungakhiqizi) okujwayelekile
|
| Cabaphikisayo | 25 isistimu ye-μl (μl) | 10 isistimu ye-μl (μl) |
| I-Master Mix | 4 × I-Master Mix VII | 6.25 | 2.5 |
| 5 x 30AI-Primer Mix | 5.0 | 2 | |
| Amanzi enziwe deionized | 13.75 | 5.5 | |
| Bukuthungatha ibala | Ububanzi 1.2mm | Ububanzi 1.2mm | |
| Tivolumu yokusabela kwe-otal | 25 μl | 10 μl | |
Ukuhlaziywa Kwedatha
Igrafu enamathiselwe 1:Imephu ye-Allelic Ladder Typing
Igrafu enamathiselwe 2:Ukuthayipha kwe-DNA okujwayelekile okungu-9948imephu
I-Reagent Storage
1. Sicela ugcine ngaphansi kokungu-20°C, ngemva kokuthola amakhithi eqhwa eqhweni elomile noma amaphakethe eqhwa ejeli.
2. Sicela ugcine ingxenye ye-pre-reaction 4×premix VII ku- -20°C, ngemva kokuthi ikhithi ikhishiwe ukuze isetshenziswe , futhi ugcine ezinye izinto ezisasabela ngaphambilini ezisele ku-4°C ukuze ugweme ukuqhwa okuphindaphindiwe nokuncibilika.Uma umthamo owodwa umncane, kunconywa ukuthi ugcinwe ku--20 ° C ngemuva kokukhishwa kwe-aliquoting,
3. Gcina izingxenye ngemva kokusabela ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ungathinti ama-reagents ngaphambi kokusabela ukuze ugweme ukungcoliswa.
-
I-Foregene DNA Identification System 27Y
-
I-Foregene DNA Identification System Y Plus (Non...
-
I-Foregene DNA Identification System 20A (I-DN Yamahhala...
-
I-Foregene DNA Identification System 25A-C (Ikesi)
-
I-Foregene DNA Identification System 38Y-C (Ikesi)
-
I-Foregene DNA Identification System 30 AC (Ikesi)
