Imbewu Yezitshalo(i-Polysaccharide Polyphenol ecebile, encane) I-Direct PCR Plus Kit I-UNG(ngaphandle Kwamathuluzi Okusampula)
Izincazelo
Lo mkhiqizo usebenzisa isistimu yokusabela ye-lysis eyingqayizivele, engakhulula ngokushesha i-DNA ye-genomic kumasampula embewu yezitshalo ane-polysaccharides ephezulu nama-polyphenols (njengobhanana, ukotini, njll.) ukuze isetshenziswe ekuphenduleni kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Ukuze kuhlangatshezwane nezidingo zokuhlolwa kokutholwa okuhlukile, le kit ingasebenzisa izinhlobo eziningi zamasampuli (njengembewu ephelele, ukusikeka kwezicubu ezincane, noma amasampula aphansi embewu eminingi) ukuze kuhlolwe.Phakathi kwazo, amasampula embewu asadinga ukuhluma, ungakhetha ukusika izingcezu zezicubu ezincane (1-5mg) ngaphandle kwemibungu yembewu ukuze uhlole;ngokuhlolwa okudinga amasampula nokuhlolwa enanini elikhulu lamasampuli, ungakhetha ukuhlanganisa amasampuli amaningi futhi uwahlanganise.Ngemva kokugaya zibe izinhlayiya ezinobubanzi obungaba ngu-0.5mm, ukuhlolwa kungenziwa (okungenani u-0.1% wesampula okuhlosiwe kungatholwa).
Imininingwane
50T,200T,500T,2000T
Izingxenye zekhithi
Plant Seed Direct PCR Plus Kit I-UNG I-Polysaccharide Polyphenol Plant Seed (Encane) Direct PCR Kit-UNG | |||||
Ukwakheka kwekhithi | I-TP-0316T | I-TP-03161 | I-TP-03162 | I-TP-03163 | |
50 T | 200 T | 500 T | 2000 T | ||
Ingxenye I | Isivimbeli SSP1 | 4.5ml | 18ml | 45ml | 90mlx2 |
Isilondolozi se-SP2 | 3ml | 10ml | 25ml | 100ml | |
I-6× I-DNA Yokulayisha Ibhafa | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Ingxenye II | Isivimbeli SSP2 | 1.5ml | 6ml | 15ml | 60ml |
2× Imbewu PCR EasyTM Mix | 500μl | 1mlx2 | 1.7ml×3 | 1.7ml×12 | |
Imanuwali | 1 | 1 | 1 | 1 |
Izici nezinzuzo
- Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo.
- Isidingo sesampula sincane, thatha nje imbewu eyodwa.
- Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukusebenza kulula.
- Uhlelo lwe-PCR olulungiselelwe lwenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
- Isistimu ye-PCR yokulwa nokungcoliswa 2× Imbewu PCR KululaTM I-Mix (UNG), engaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukucaciswa nokunemba kokukhulisa.
Ukuhamba komsebenzi
Izimo zokugcina
1.Ezokuthutha
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi Ingxenye II isesimweni esingu-<4 ℃.
2.Isitoreji
Ingxenye I yale kit kufanele igcinwe ekamelweni lokushisa noma 2-8 ° C.
Ingxenye II kufanele igcinwe ku -20°C.