I-Serum(Plasma) miRNA Isolation Kit
Incazelo yekhithi:
◆Isethi egcwele yekhithi i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
◆Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
◆Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngempumelelo.
◆Isivinini esisheshayo: Ukusebenza kulula futhi kungaqedwa phakathi nemizuzu engama-30.
◆Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
◆Ikhwalithi ephezulu: Izingcezu ze-RNA ezikhishiwe zinokuhlanzeka okuphezulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokusebenza zokuhlola ezehla nomfula.
Izincazelo
Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe inkampani yethu, engakhipha kahle izingcezwana ezincane ze-miRNA ngaphansi kuka-100nt ku-serum ne-plasma.Ikhithi inikeza ikholomu ye-DNA-Cleaning, engahlukanisa kalula i-supernatant ne-tissue lysate ne-adsorb futhi isuse i-DNA kusampula, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa i-RNA ngokuphumelelayo, futhi ngefomula ehlukile, ingasetshenziswa ngesikhathi esifanayo.Lungiselela inani elikhulu lamasampuli.
Lonke uhlelo alunayo i-RNase-Free, ukuze i-miRNA ehlanziwe ingonakalisiwe;I-Buffer miBRW1 kanye ne-Buffer miBRW2 buffer system yokugeza yenza i-RNA itholwe ngaphandle kokungcola kweprotheyini, i-DNA, i-ion, kanye nenhlanganisela yezinto eziphilayo.
Imininingwane
5T, 50T, 100T, 200T
Izingxenye zekhithi
| I-Serum/Plasma miRNA Isolation Kit | ||||
| Okuqukethwe kwekhithi | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| I-ddH yamahhala ye-RNase2O | 1.7ml | 10ml | 20ml | 40ml |
| Ikholomu ye-RNA kuphela | 5 izingcezu | 50izingcezu | 100izingcezu | 200izingcezu |
| Ikholomu Yokuhlanza I-DNA | 5 izingcezu | 50izingcezu | 100izingcezu | 200izingcezu |
| IFU | 1 ucezu | 1 ucezu | 1 ucezu | 1 ucezu |
Izici&izinzuzo
◆Isethi egcwele yekhithi i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
◆Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
◆Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngempumelelo.
◆Isivinini esisheshayo: Ukusebenza kulula futhi kungaqedwa phakathi nemizuzu engama-30.
◆Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
◆Ikhwalithi ephezulu: Izingcezu ze-RNA ezikhishiwe zinokuhlanzeka okuphezulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokusebenza zokuhlola ezehla nomfula.
Imingcele yekhithi
Isicelo sekhithi
Ilungele ukukhishwa nokuhlanzwa kwe-miRNA ku-serum yezilwane neyomuntu kanye ne-plasma.
Isicelo se-miRNA
I-miRNA ehlanzwe yi-Serum/Plasma miRNA Isolation Kit ingasetshenziswa ekuhloleni okuhlukahlukene kwamangqamuzana angezansi komfula, okufana nalokhu: RT-PCR, Isikhathi Sangempela RT-PCR, I-Northern Blot, ukuhlaziywa kwe-chip, njll.
Ukugeleza komsebenzi
Izimo zokugcina
Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa(15-25°C).Iuma idinga ukugcinwa isikhathi eside,Ngiyacelaemazingeni okushisa angama-2-8 ° C.
