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Okuphambili kokutholakala kwezidakamizwa: Ukukhomba i-RNA ngamamolekyuli amancane
Umuthi wokugomela i-mRNA we-Pfizer we-COVID usuvuselele uthando lokusebenzisa i-ribonucleic acid (RNA) njengendlela yokwelapha.Kodwa-ke, ukukhomba i-RNA ngama-molecule amancane kuyinselele enkulu.I-RNA inamabhulokhi wokwakha amane kuphela: i-adenine (A), i-cytosine (C), i-guanine (G), ne-uracil (U) ethatha indawo ye-thymin...Funda kabanzi -
Yini izidakamizwa ze-nucleic acid?
“Izidakamizwa ze-Nucleic acid” zisebenzisa i-“nucleic acid,” ebhekisela ezintweni ezinjenge-DNA ne-RNA ezilawula ukwaziswa kofuzo, njengezidakamizwa.Lokhu kuvumela ukukhonjwa kwama-molecule afana ne-mRNA ne-miRNA engakwazi ukuqondiswa ngezidakamizwa zendabuko ezinesisindo esiphansi semolekyuli nemithi yamasosha omzimba, futhi kukhona i-g...Funda kabanzi -
Olunye ulwazi mayelana ne-siRNA ku-RT-PCR
1. Ulwazi oluyisisekelo (uma ufuna ukubona ingxenye yokuhlola, sicela udlulisele ngqo engxenyeni yesibili) Njengokusabela kokususelwe kwe-PCR evamile, I-PCR yesikhathi sangempela iqapha ngokuyinhloko ukuguqulwa kwenani lomkhiqizo wokukhulisa umjikelezo kumjikelezo ngamunye wokukhulisa i-PCR. ukusabela ngesikhathi sangempela thr...Funda kabanzi -
Isifinyezo esinemininingwane yesistimu yokusabela yokuhlola ye-RT-PCR
一、Khulisa ukuzwela kwesistimu yokusabela: 1. Hlukanisa i-RNA yekhwalithi ephezulu: Ukuhlanganiswa kwe-cDNA okuphumelelayo kuvela ku-RNA yekhwalithi ephezulu.I-RNA yekhwalithi ephezulu kufanele okungenani ibe ubude obugcwele futhi ingabi nama-reverse transcriptase inhibitors njenge-EDTA noma i-SDS.Izinga le-RNA linquma ubukhulu be-a...Funda kabanzi -
Qedela idizayini yokuqala ye-PCR kanye nemininingwane ye-PCR
Isisekelo sokuklama kokuqala (izinkinga ezingu-99% zingaxazululwa) 1. Ubude be-primer: Incwadi yokufunda idinga u-15-30bp, ngokuvamile mayelana no-20bp.Isimo sangempela singcono sibe ngu-18-24bp ukuze kuqinisekiswe ukucaciswa, kodwa isikhathi esingcono, i-primer ende kakhulu izonciphisa ukucaciswa, futhi inciphise isivuno.2. Prim...Funda kabanzi -
Izinyathelo zokuphepha ze-qRT-PCR ezihlanganiswe nguDokotela omkhulu ngobuhlakani
Wonke umuntu ukhuluma ngomgomo wokuhlolwa kwe-qRT-PCR, ukuklama kokuqala, ukuchazwa kwemiphumela, njll., kodwa ngicabanga ukuthi kufanele ngabelane nawe ngokusebenza kokuhlola kwe-qRT-PCR.Incane, kodwa imayelana nemiphumela.Ngaphambi kokwenza i-qRT-PCR, sidinga ukuqonda ngokucacile ...Funda kabanzi -
Izinyathelo zokuphepha ze-RT-qPCR
Ukuhlolwa kwe-RT-qPCR kufaka phakathi ukukhipha kwe-RNA nokuhlolwa kwekhwalithi, ukuloba okuhlanekezelwe kanye nezinyathelo ezintathu ze-qPCR, isinyathelo ngasinye sinezinyathelo eziningi zokuphepha, sizokwethula ngokuningiliziwe ngezansi.Ⅰ.Ukuhlolwa kwekhwalithi ye-RNA Ekuhlolweni kwe-RT-qPCR, ngemva kokuqedwa kokukhipha i-RNA, ...Funda kabanzi -
Isingeniso esiphelele se-PCR yesikhathi sangempela
1. Ukuqonda kwasekuqaleni Kulesi sigaba, sidinga ukuqonda imiqondo ethile namagama, ukuze sigweme ukwenza amaphutha phambi kwabadala bethu, njengokuthi: Q: Uyini umehluko phakathi kwe-RT-PCR, qPCR, Real-time PCR, kanye ne-RT-PCR yesikhathi sangempela?Impendulo: I-RT-PCR iwukuhlanekezela okulotshiweyo kwe-PCR (reverse t...Funda kabanzi -
I-PCR, i-PCR eminingi, i-In situ PCR, i-Reverse PCR, i-RT-PCR, i-qPCR (1)- PCR
I-PCR, i-PCR eminingi, In situ PCR, Reverse PCR, RT-PCR, qPCR(1)– PCR Sizohlunga imiqondo, izinyathelo kanye nemininingwane ye-PCR ehlukahlukene Ⅰ.I-PCR Polymerase Chain Reaction, ebizwa ngokuthi i-PCR, ubuchwepheshe bebhayoloji yamangqamuzana obusetshenziselwa ukukhulisa izingcezu ezithile ze-DNA.Kungathathwa njenge...Funda kabanzi -
Umgomo wokuhlola we-qRT-PCR
I-RT-qPCR ithuthukiswe kusukela kubuchwepheshe obujwayelekile be-PCR.Yengeza amakhemikhali e-fluorescent (odayi be-fluorescent noma ama-fluorescent probes) kusistimu yokusabela ye-PCR evamile, futhi ithola inqubo yokukhishwa kwe-PCR nesandiso ngesikhathi sangempela ngokuya ngezinqubo zabo zokukhanya ezihlukile.Isignali ye-Fluorescent...Funda kabanzi -
Isifinyezo esinemininingwane yesistimu yokusabela yokuhlola ye-RT-PCR
Ⅰ.Khulisa ukuzwela kwesistimu yokusabela: 1. Hlukanisa i-RNA yekhwalithi ephezulu: Ukuhlanganiswa kwe-cDNA okuphumelelayo kuvela ku-RNA yekhwalithi ephezulu.I-RNA yekhwalithi ephezulu kufanele iqinisekise okungenani ingqikithi ende futhi ayiqukethe ama-inhibitor anganawo ama-enzyme okurekhoda, njenge-EDTA noma i-SDS.I-quali...Funda kabanzi -
Ungaqinisekisa Kanjani Ibanga Elinengqondo Lamanani E-CT Okuhlolwa Kwe-qPCR
Inani le-CT liwuhlobo lokwethula umphumela obaluleke kakhulu we-PCR ye-fluorescent quantitative.Isetshenziselwa ukubala umehluko wokuchazwa kofuzo noma inombolo yekhophi yofuzo.Ngakho-ke liyini inani le-Ct le-fluorescence quantification elithathwa njengelinengqondo?Ungaqinisekisa kanjani ibanga elisebenzayo levelu ye-CT?Yini Ct...Funda kabanzi
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