I-China PCR Heroᵀᴹ (Ngodayi) Umkhiqizi Nomhlinzeki |I-Foregene

I-PCR Heroᵀᴹ (With Dye)

I-PCR Heroᵀᴹ (Ngodayi) Isithombe Esifakiwe

Incazelo yekhithi:

◮Ukwethembeka okuphezulu: izikhathi ezi-6 kune-enzyme ye-Taq evamile;

◮Isivinini sokukhulisa ngokushesha

◮Ukuvumelana nezimo kwesifanekiso

◮Ukusebenza kahle kwe-amplification ephezulu

◮Ukubekezelelana kwemvelo kunamandla: kubekwe ku-37 ° C ngesonto, kugcina umsebenzi ongaphezu kuka-90%;

◮Inomsebenzi we-DNA polymerase ongu-5'→3' kanye nomsebenzi we-exonuclease ongu-5'→3', ngaphandle komsebenzi we-exonuclease ongu-3'→5'.

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane

5ml, 10ml, 40ml

I-PCR Hero (Nge-Dye) Ikhithi yokukhipha ihlanganisa isizukulwane esisha esiyingqayizivele sika-Foregene se-Taq—Foregene Taq Hero, ama-dNTP athuthukisiwe, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer nesiqinisi.Uhlelo lwe-2× PCR Hero TM Mix (udayi) lunokubekezelela okuphezulu kwe-PCR inhibitors kunohlelo olujwayelekile lwe-PCR Mix, futhi lungabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.

Izingxenye zekhithi

0.1U Taq Hero/μl

400μI-M dNTPs

20mM Tris-HCl(pH8.8)

100mM KCl

5mM MgCl2

Amanye ama-stabilizer kanye nezithuthukisi

I-DNA Loading Buffer

Izici&izinzuzo

■Ukwethembeka okuphezulu: izikhathi ezi-6 kune-enzyme ye-Taq evamile;

■ Isivinini sokukhulisa esisheshayo : ijubane lokukhulisa ngu-5-10sec/kb, eliphindwe izikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;

■ Ukuguquguquka okwengeziwe kwesifanekiso: Ingakwazi ukukhulisa izifanekiso ze-DNA eziyinkimbinkimbi ngokuphezuluOkuqukethwe kwe-GC;

■ Ukusebenza okuphakeme kokukhulisa amandla: imijikelezo yokukhulisa ephansi kune-enzyme ye-Taq evamile;

■ Ukubekezelela imvelo kunamandla: kubekwe ku-37°C ngesonto, ukugcina umsebenzi ongaphezu kwama-90%;

■ Ino-5'→3'Umsebenzi we-DNA polymerase kanye ne-5'→3'umsebenzi we-exonuclease, ngaphandle kwe-3'→5'umsebenzi we-exonuclease.

■ Ikhithi ixutshwe ngaphambili ne-Electrophoresis Loading Buffer, ngemva kokuba i-PCR isiqediwe, isampula ingalayishwa ngokuqondile i-electrophoresis;ukusebenza kulula futhi kulula.