I-SARS-CoV-2 Nucleic Acid Detection Kit (I-Multiplex PCR Fluorescent Probe Method)

Incazelo yekhithi:

 

◮ I-PCR eqondile:Asikho isidingo sekhithi yokukhipha i-nucleic acid kanye neSistimu, iqede isifanekiso esingu-96 ngehora elingu-1 noma ngaphezulu.

 

◮ Isidingo semishini ephansi kanye nokusetshenziswa okuvumelana nezimo kakhulu:Kudingeka kuphela isistimu ye-PCR Yesikhathi Sangempela .

 

◮ I-LoD nokuzwela okuphezulu:Ukuzwela kokutholwa kungafinyelela ezingeni eliphansi kakhulu njengamakhophi angama-500/ml.

foregene strength


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ukusetshenziswa Okuhlosiwe

Le khithi isebenzisa ubuchwepheshe be-Real-time RT PCR (rRT-PCR) ukuthola ikhwalithi yama-nucleic acid akwa-SARS-CoV-2 kumasampula e-nasopharyngeal noma e-oropharyngeal swab.

Isimiso sokuhlola

Lesi siqalisi kanye namasethi ophenyo aklanyelwe ukutholwa kokulandelana okulondoloziwe kwe-SARS-CoV-2 (ORF1ab gene kanye nofuzo lwe-N).Ikhithi iqukethe Ukulawulwa Kwangaphakathi okusetshenziselwa ukuhlola ikhwalithi yesifanekiso.
Ubuchwepheshe be-PCR besikhathi sangempela busebenzisa i-polymerase chain reaction (PCR) ukuze kukhuliswe ithagethi ethile.
ukulandelana futhi kuqondise ama-probe athile ukuze kutholwe i-RNA ekhulisiwe.Ama-probe abhalwe ngokuthi
intatheli ye-fluorescent kanye namadayi wokucisha.

Imininingwane Esheshayo

Imininingwane:48 Rxns/kit, 96 Rxns/kit

◮ Ukucaciswa:96.72%

Ukuzwela:97.92%

◮Izinhlobo zesifanekiso esisebenzayo
I-Nasopharyngeal Swab noma i-Oropharyngeal Swab
Ithuluzi elisebenzayo
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

 

Izingxenye

Cha.

Isakhi

Inani

Izingxenye eziyinhloko

awu 48r

awu 96r

1 I-ejenti yokukhululwa kwe-Nucleic acid 1.4mL/ithubhu 2 amashubhu 5.3 mL/ibhodlela 1 ibhodlela I-surfactant
2 Isivikeli se-RNA 27 μL/ithubhu 1 ithubhu 53 μL/ithubhu 1 ithubhu I-RNase inhibitor
3 Isixazululo sokusabela sakwa-SARS-CoV-2 800 μL / ishubhu 1 ithubhu 1600 μL/ipayipi 1 ithubhu I-Primer, probe, buffer yokusabela, i-dNTP
4 Ingxube ye-enzyme ye-SARS-CoV-2 80 μL/ipayipi 1 ithubhu 160 μL/ishubhu 1 ithubhu Isiqalo esishisayo se-Taq enzyme, i-enzyme ye-M-MLV
5 Ukulawula okuhle kwe-SARS-CoV-2 100 μL / ishubhu 1 ithubhu 100 μL / ishubhu 1 ithubhu I-plasmid ehlanganisiwe equkethe ingxenye yethagethi, i-RNA
6 Ukulawula okungekuhle kwe-SARS-CoV-2 1200 μL / ishubhu 1 ithubhu 1200 μL / ishubhu 1 ithubhu Ibhafa ye-TE

Izici&izinzuzo

I-PCR eqondile
Asikho isidingo sekhithi yokukhipha i-nucleic acid kanye neSistimu, iqede isifanekiso esingu-96 ngehora elingu-1 noma ngaphezulu.
Isidingo semishini ephansi kanye nokusetshenziswa okuguquguqukayo kakhulu
Kudingeka kuphela isistimu ye-PCR Yesikhathi Sangempela.
I-LoD nokuzwela okuphezulu
Ukuzwela kokutholwa kungafinyelela ezingeni eliphansi kakhulu njengamakhophi angama-500/ml.
amplification curve-RT-qPCR

Isetshenziswa kanjani

Indlela 1: Indlela ye-PCR eqondile

Indlela 2: I-Viral RNA Isolation + PCR

Indlela yoku-1: Indlela ye-PCR eqondile usebenzisaUmenzeli Wokukhishwa Kwesampula  

 

Inqubo yokuthola

Operation Procedures

Procedures

I-Cold Chain Ezokuthutha kanye Nesitoreji

Ivalwe ekukhanyeni futhi igcinwe ku -20±5℃;

storage
storage2

Impilo yeshelufu

1 unyaka

Uku-oda Ulwazi

Ordering information

Ividiyo yokusebenza okusheshayo


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