I-SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

I-SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

Incazelo Yekhithi:

Leli thuluzi lisebenzisa ubuchwepheshe be-Real-time RT PCR technology (rRT-PCR) ukuthola ukutholakala kwekhwalithi ye-SARS-CoV-2 (ORF1ab gene kanye no-N gene) ama-nucleic acid kumasampula womuntu we-nasopharyngeal noma we-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukulawula kwangaphakathi ukuhlola ikhwalithi yesifanekiso, ngakho-ke asikho isidingo sokuhlanzwa kwe-nucleic acid, okungaqedela ukuhlolwa ngehora eli-1, ikakhulukazi elifanele ukutholwa okusheshayo okukhulu.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Imininingwane

Ama-Rxns angama-48, ama-Rxns angama-96

Cha.

Ingxenye

Inani

Izingxenye eziyinhloko

Ama-Rxns angama-48

Ama-Rxns angama-96

1 Umenzeli wokukhishwa kwe-Nucleic acid 1.4mL / ithubhu 2 amashubhu 5.3 ml / ibhodlela Ibhodlela elilodwa I-Surfactant
2 Ukuvikelwa kwe-RNA 27 μL / ithubhu 1 ithubhu 53 μL / ithubhu 1 ithubhu I-RNase inhibitor
3 Isixazululo sokuphendula se-SARS-CoV-2 800 μL / ithubhu 1 ithubhu 1600 μL / ithubhu 1 ithubhu I-Primer, probe, i-reaction buffer, i-dNTP
4 Inhlanganisela ye-enzyme ye-SARS-CoV-2 80 μL / ithubhu 1 ithubhu 160 μL / ithubhu 1 ithubhu Ukuqala okushisayo Taq enzyme, M-MLV enzyme
5 Ukulawulwa okuhle kwe-SARS-CoV-2 100 μL / ithubhu 1 ithubhu 100 μL / ithubhu 1 ithubhu I-plasmid ephindayo equkethe isiqeshana sethagethi, i-RNA
6 Ukulawulwa okungalungile kwe-SARS-CoV-2 1200 μL / ithubhu 1 ithubhu 1200 μL / ithubhu 1 ithubhu I-TE buffer

Izici nezinzuzo

Kuyashesha futhi kulula.
Gwema ukulahleka kwe-nucleic acid.
Umkhawulo wokubona ophansi nokuzwela okuphezulu.
Isidingo semishini ephansi nokusetshenziswa okuguqukayo kakhulu.

Ukuzwela kokutholwa kungafinyelela ezingeni eliphansi kakhulu njengamakhophi ayi-500 / mL).

I-GCE / mL Inani le-Ct
Uhlobo luka-N Uhlobo lwe-ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 Akunjalo Akunjalo
2.00E-01 Akunjalo Akunjalo
NC Akunjalo Akunjalo

Umugqa wokukhulisa ijika

amplification curve-RT-qPCR

Isetshenziswa kanjani

1. Insimbi esebenzayo

covid-19 nucleic acid detection kit1

2. Inqubo yokuthola

covid-19 nucleic acid detection kit2

Ukucutshungulwa kwesampula: Isampula ye-Naopharyngeal noma ye-oropharyngeal swab (ukusebenza kwesinyathelo esingu-1 kuphela) (Linda imizuzu eyi-10)

I-PCR amplificaton (<= 55min)

Ukuhamba komsebenzi:

covid-19 nucleic acid detection kit4

Isitoreji

ivaliwe kusuka ekukhanyeni futhi igcinwe ku- -20 ± 5 ℃;

storage
storage2

Impilo eshalofini: 1 unyaka


  • Langaphambilini
  • Olandelayo:

  • Bhala umyalezo wakho lapha bese uwuthumela kithi