I-SARS-CoV-2 Nucleic Acid Detection Kit (I-Multiplex PCR Fluorescent Probe Method)
Ukusetshenziswa Okuhlosiwe
Le kit isebenzisa ubuchwepheshe be-Real-time RT PCR (rRT-PCR) ukuthola ikhwalithi yama-nucleic acid akwa-SARS-CoV-2 kumasampula e-nasopharyngeal noma e-oropharyngeal swab.
Isimiso sokuhlola
Lesi siqalisi kanye namasethi ophenyo aklanyelwe ukutholwa kokulandelana okulondoloziwe kwe-SARS-CoV-2 (ORF1ab gene kanye nofuzo lwe-N).Ikhithi iqukethe Ukulawulwa Kwangaphakathi okusetshenziselwa ukuhlola ikhwalithi yesifanekiso.
Ubuchwepheshe be-PCR besikhathi sangempela busebenzisa i-polymerase chain reaction (PCR) ukuze kukhuliswe ithagethi ethile.
ukulandelana futhi kuqondise ama-probe athile ukuze kutholwe i-RNA ekhulisiwe.Ama-probe abhalwe ngokuthi
intatheli ye-fluorescent kanye namadayi wokucisha.
Imininingwane Esheshayo
◮Imininingwane:48 Rxns/kit, 96 Rxns/kit
◮ Ukucaciswa:96.72%
Ukuzwela:97.92%
◮Izinhlobo zesifanekiso esisebenzayo
I-Nasopharyngeal Swab noma i-Oropharyngeal Swab
◮Ithuluzi elisebenzayo
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Izingxenye
Cha. | Isakhi | Inani | Izingxenye eziyinhloko | |||
awu 48r | awu 96r | |||||
1 | I-ejenti yokukhululwa kwe-Nucleic acid | 1.4mL/ithubhu | 2 amashubhu | 5.3 mL/ibhodlela | 1 ibhodlela | I-surfactant |
2 | Isivikeli se-RNA | 27 μL/ithubhu | 1 ithubhu | 53 μL/ithubhu | 1 ithubhu | I-RNase inhibitor |
3 | Isixazululo sokusabela sakwa-SARS-CoV-2 | 800 μL / ishubhu | 1 ithubhu | 1600 μL/ipayipi | 1 ithubhu | I-Primer, probe, buffer yokusabela, i-dNTP |
4 | Ingxube ye-enzyme ye-SARS-CoV-2 | 80 μL/ithubhu | 1 ithubhu | 160 μL/ishubhu | 1 ithubhu | Isiqalo esishisayo se-Taq enzyme, i-enzyme ye-M-MLV |
5 | Ukulawula okuhle kwe-SARS-CoV-2 | 100 μL / ishubhu | 1 ithubhu | 100 μL / ishubhu | 1 ithubhu | I-plasmid ehlanganisiwe equkethe ingxenye yethagethi, i-RNA |
6 | Ukulawula okungekuhle kwe-SARS-CoV-2 | 1200 μL / ishubhu | 1 ithubhu | 1200 μL / ishubhu | 1 ithubhu | Ibhafa ye-TE |
Izici&izinzuzo
◮I-PCR eqondile
Asikho isidingo sekhithi yokukhipha i-nucleic acid kanye neSistimu, iqede isifanekiso esingu-96 ngehora elingu-1 noma ngaphezulu.
◮Isidingo semishini ephansi kanye nokusetshenziswa okuguquguqukayo kakhulu
Kudingeka kuphela isistimu ye-PCR Yesikhathi Sangempela.
◮I-LoD nokuzwela okuphezulu
Ukuzwela kokutholwa kungafinyelela ezingeni eliphansi kakhulu njengamakhophi angu-500/ml.
Isetshenziswa kanjani
Indlela yoku-1: Indlela ye-PCR eqondile
Indlela 2: I-Viral RNA Isolation + PCR
Indlela 1: Indlela ye-PCR eqondile usebenzisaUmenzeli Wokukhishwa Kwesampula
I-Cold Chain Ezokuthutha kanye Nesitoreji
Ivalwe ekukhanyeni futhi igcinwe ku -20±5℃;
Impilo yeshelufu
1 unyaka
Uku-oda Ulwazi
Ividiyo Yokusebenza Okusheshayo
Bhala umyalezo wakho lapha futhi usithumelele wona