I-Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix ye-Genotyping of Blood
Incazelo yekhithi:
Ngokuqondile sebenzisa igazi eliphelele le-EDTA le-anticoagulated njengesifanekiso sokukhulisa i-PCR, esinokuzwela okuphezulu nokusebenza kahle kokukhulisa amandla.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Asikho isidingo sokuhlanzwa kwe-DNA okuthatha isikhathi esiningi nokubizayo, akukho ukwelashwa kusengaphambili, nokusetshenziswa okuqondile kwegazi eliphelele njengesifanekiso se-PCR.
◮Isistimu inekhono eliqinile lokukhulisa futhi ingakwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
◮2 × SuperEasyTMI-Mix(UNG)-EDTA inokubekezelela igazi okuphezulu: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
◮Isampula livalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.Uhlelo lwe-PCR olulwa nokungcola
◮2 × SuperEasyTMI-Mix(UNG)-EDTA, iqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR, futhi iqinisekisa ukucaciswa nokunemba kokukhulisa.
Imininingwane
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMImiksi(UNG)-EDTA :Ngesisekelo se-2×SuperEasyTMI-Mix-EDTA, i-dUTP isetshenziswa esikhundleni se-dTTP, futhi i-enzyme ye-UNG engehlisa isithunzi isifanekiso esiqukethe i-dUTP yengezwa ngesikhathi esifanayo, engavimbela ngempumelelo ukungcoliswa kwemikhiqizo yokukhulisa i-PCR.Iqukethe i-D-Taq DNA Polymerase elungiswe ngokukhethekile, i-dNTPs, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer ne-stabilizer, njll. Ekuphenduleni kwe-PCR, vele wengeze igazi eliphelele elinqanda ukucoagulate, ama-primer, ne-ddH.2O ku-2× SuperEasy ehambisanayoTMI-Mix(UNG)-EDTA izosetshenziswa ekuphenduleni kwe-PCR.
I-6× I-DNA Loading Buffer:I-Buffer Yokulayisha ayinayo i-SDS.Kunconywa ukusebenzisa i-6× DNA Loading Buffer efakwe nekhithi uma wenza i-agarose gel electrophoresis ukuze uthole imiphumela emihle ye-electrophoresis.Ungasebenzisi i-Loading Buffer equkethe i-SDS, ngaphandle kwalokho kuzoba nomsila omkhulu wokukhanya okukhanyayo emzileni ngesikhathi se-electrophoresis, okuzothinta imiphumela yokuhlola.
Izingxenye zekhithi
| 2 × SuperEasyTMImiksi(UNG)-EDTA |
| I-6× I-DNA Yokulayisha Ibhafa |
| Iziyalezo |
Izici&izinzuzo
- Asikho isidingo sokuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo, akukho ukwelashwa kusengaphambili, nokusetshenziswa okuqondile kwegazi eliphelele njengesifanekiso se-PCR.
-Isistimu inekhono eliqinile lokukhulisa futhi ikwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
-2 × SuperEasyTMI-Mix(UNG)-EDTA inokubekezelela igazi okuphezulu: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
-Isampula ivalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.Uhlelo lwe-PCR olulwa nokungcoliswa kwe-2 × SuperEasyTMI-Mix(UNG)-EDTA, iqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR, futhi iqinisekisa ukucaciswa nokunemba kokukhulisa.
Isicelo sekhithi
Kunikezelwe ekuhlonzeni okuqondile kwe-PCR ye-EDTA egazini eligcwele igazi.(Kuhlanganisa: igazi lomuntu, igazi legundane, igazi lenkukhu, igazi lenyoni, igazi lenkomo, igazi lenja, njll.)
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Le kit igcinwa ku -20°C.
2 × SuperEasyTMI-Mix(UNG)-EDTA，Uma isetshenziswa njalo, ingabuye igcinwe ku-4°C ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).
I-6× DNA Loading Buffer, Ingagcinwa ku-4°C noma -20°C isikhathi eside.
