• facebook
  • linkedin
  • youtube
English
isibhengezo_sekhasi

I-Foregene DNA Identification System Y Plus (Engakhishiwe)

I-Foregene DNA Identification System Y Plus ( Engakhishiwe) Isithombe Esifakiwe
  • I-Foregene DNA Identification System Y Plus (Engakhishiwe)
  • I-Foregene DNA Identification System Y Plus (Engakhishiwe)
  • I-Foregene DNA Identification System Y Plus (Engakhishiwe)
  • I-Foregene DNA Identification System Y Plus (Engakhishiwe)

Incazelo yekhithi:

I-ForegeneIsistimu yokuhlonza i-DNA i-Y Plus isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ukuze ikhulise i-20 Y chromosome core loci, 15 loci ekhethwayo, 6 alternative loci kanye 3 Y indel loci ngesikhathi esisodwa..

amandla angaphambili


Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

I-ForegeneIsistimu yokuhlonza i-DNA i-Y Plus isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ukuze ikhulise i-20 Y chromosome core loci, 15 loci ekhethwayo, 6 alternative loci kanye 3 Y indel loci ngesikhathi esisodwa..

Okuqukethwe kwekhithi

Ukulungiswa kwesistimu yokukhulisa

Iukwakheka kokulungiselelakwe-sejwayelekile(ngaphandle kokukhipha)uhlelo lokukhulisa amandla

 

Cabaphikisayo

25 μluhlelo (μl)

10μl uhlelo (μl)

I-Master Mix 

4 × I-Master MixVII

6.25

2.5

5×Y Plus Primer Mix

5.0

2.0

Amanzi enziwe deionized

13.75

5.5

Bukuthungatha ibala

Ububanzi 1.2mm

Ububanzi 1.2mm

Tivolumu yokusabela kwe-otal

25 μl

10 μl

Ukuhlaziywa Kwedatha

Igrafu enamathiselwe 1:Imephu ye-Allelic Ladder Typing

Igrafu enamathiselwe 2:Ukuthayipha kwe-DNA okujwayelekile okungu-9948imephu

I-Reagent Storage

1. Sicela ugcine ngaphansi kuka -20°C(Uma ingasetshenziswanga okwesikhashana),ngemuva kokuthola amakhithi eqhwa eqhweni elomile noma amaphakethe eqhwa ejeli.

2. Sicela ugcine ingxenye ye-pre-reaction 4×premix VII ku--20°C, ngemva kokuba ikhithi isikhishiwe ukuze isetshenziswe, kanye nezinye izinto ezisasabela ngaphambilini ezisele zigcinwa ku-4°C ukugwema ukuqhwa okuphindaphindiwe nokuncibilika.Uma umthamo owodwa umncane, kunconywa ukuthi ugcinwe ku -20 ° C ngemuva kokukhipha i-aliquoting.

3. Gcina izingxenye ngemva kokusabela ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ungathinti ama-reagents ngaphambi kokusabela ukuze ugweme ukungcoliswa.

  • Okwedlule:
  • Olandelayo:
    • Bhala umyalezo wakho lapha futhi usithumelele wona

    OkuhlobeneImikhiqizo

    Chofoza u-enter ukuze useshe noma u-ESC ukuze uvale