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I-Foregene DNA Identification System 30 AC (Ikesi)

I-Foregene DNA Identification System 30 AC (Ikesi) Isithombe Esifakiwe
  • I-Foregene DNA Identification System 30 AC (Ikesi)
  • I-Foregene DNA Identification System 30 AC (Ikesi)
  • I-Foregene DNA Identification System 30 AC (Ikesi)
  • I-Foregene DNA Identification System 30 AC (Ikesi)

Incazelo yekhithi:

I-Foregene Uhlelo lokuhlonza i-DNA30A-U-C usebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuzekhulisa 29i-autosomalSTR indawo, 1 I-Amel locus, i-1 Yindellocus, futhi1 Izingosi zezocansi eziqondene nezinhlobo zabantu, 2amasayithi okulawula ikhwalithi angaphakathi, asetshenziselwa ukukhulisaI-DNAisifanekiso esikhishwe futhi sahlanzwa kusoCizinto zokwakha.

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Incazelo

I-Foregene Uhlelo lokuhlonza i-DNA30A-U-C usebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuzekhulisa 29i-autosomalSTR indawo, 1 I-Amel locus, i-1 Yindellocus, futhi1 Izingosi zezocansi eziqondene nezinhlobo zabantu, 2amasayithi okulawula ikhwalithi angaphakathi, asetshenziselwa ukukhulisaI-DNAisifanekiso esikhishwe futhi sahlanzwa kusoCizinto zokwakha.

Okuqukethwe kwekhithi

Ukulungiswa kwesistimu yokukhulisa

Ithebula 2: Ukwakhiwa Kokulungiselelwa Kokukhishwa Okujwayelekile kanye nesistimu yokukhulisa izwi

 

Cabaphikisayo

25ul uhlelo (µl)

10μl uhlelo (μl)

I-Master Mix

 2.5× I-PremixCⅡ

10.0

4.0
5 x 30 I-AC Primer Mix

5.0

2.0
Isifanekiso se-DNA

2.5-10

1-4
Amanzi enziwe deionized

Yenza ivolumu yokusabela ibe ngu-25μl

Yenza ivolumu yokusabela ye10 ml

Ukuhlaziywa Kwedatha

Igrafu enamathiselwe 1:Imephu ye-Allelic Ladder Typing

Igrafu enamathiselwe 2:Ukuthayipha kwe-DNA okujwayelekile okungu-9948imephu

I-Reagent Storage

1. Sicela ugcine ngaphansi kokungu-20°C, ngemva kokuthola amakhithi eqhwa eqhweni elomile noma amaphakethe eqhwa ejeli.

2. Sicela ugcine ingxenye ye-pre-reaction engu-2.5× premix CII ku--20°C ngemva kokuthi ikhithi ikhishiwe ukuze isetshenziswe, futhi ama-reagents asele asabela ngaphambilini agcinwa ku-4°C ukugwema ukuqhwa okuphindaphindiwe nokuncibilika.Uma umthamo owodwa umncane, kunconywa ukuthi ugcinwe ku -20 ° C ngemuva kokukhipha i-aliquoting.

3. Gcina izingxenye ngemva kokusabela ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ungathinti ama-reagents ngaphambi kokusabela ukuze ugweme ukungcoliswa.

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