I-Foregene DNA Identification System 20A (Isizinda Se-DNA Yamahhala)
Incazelo yekhithi:
Isistimu yokuhlonza i-Foregene DNA 20A isebenzisa ubuchwepheshe bokulebula obunemibala emihlanu ukuze ngesikhathi esisodwa ikhulise i-19 STR loci kanye ne-loci yocansi engu-1 eshubhuni elilodwa.Ngenkathi ihlanganisa i-13 CODIS core loci, 20 core loci ihlangabezana nezimfuneko zoMnyango Wezokuphepha Komphakathi zekhithi ye-autosomal STR.Uyindoda engakwesokudla ye-forensic DNA yokwakhiwa kwesizindalwazi kanye nokuhlonza ukuhlobana kwezomthetho.
Incazelo
Isistimu yokuhlonza i-Foregene DNA 20A isebenzisa ubuchwepheshe bokulebula obunemibala emihlanu ukuze ngesikhathi esisodwa ikhulise i-19 STR loci kanye ne-loci yocansi engu-1 eshubhuni elilodwa.Ngenkathi ihlanganisa i-13 CODIS core loci, 20 core loci ihlangabezana nezimfuneko zoMnyango Wezokuphepha Komphakathi zekhithi ye-autosomal STR.Uyindoda engakwesokudla ye-forensic DNA yokwakhiwa kwesizindalwazi kanye nokuhlonza ukuhlobana kwezomthetho.
Okuqukethwe kwekhithi
| I-Kit Componet | Igama lenhlanganisela | Ivolumu (μl / tube) | Ubuningi (ithubhu) |
| I-PCR Pre-reaction reagent | 2.5×I-PCR yebhafa yokusabela Ⅲ | 1000 | 2 |
| 5×20A inhlanganisela yokuqala | 500 | 2 | |
| Lawula i-DNA 9947A (1ng/μl) | 25 | 1 | |
| Amanzi enziwe deionized | 1700 | 2 | |
| I-Taq Polymerase | I-Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reagent reagent | I-Allelic ladder20A | 40 | 1 |
| Isisindo samangqamuzana angaphakathi standardORG 500 | 150 | 2 | |
| Qaphela | |||
Ukuhlaziywa Kwedatha
1. Vula isofthiwe ye-ID ye-Genemapper.Lapho usebenzisa leli khithi okokuqala, udinga ukungenisa amaphaneli, i-Bin Set, indlela ehambisanayo yokuhlaziywa, usayizi ophansi (u-Org500: 60, 25, 300, 30, 30, 300, 390, 450, 490, 400)
2. Ngenisa idatha ye-electrophoresis, khetha imingcele yokuhlaziya ehambisanayo njengephaneli, indlela yokuhlaziya, nezinga lesayizi, futhi ushintshe uhlobo lwesampula Lwesitebhisi lube "I-Allelic Ladder" kukholamu "Yohlobo Lwesampula";qala ukuhlaziya idatha.
I-Reagent Storage
1. Ngemva kokuthola ikhithi efriziwe eqhweni elomile noma emaphaketheni eqhwa epulasitiki, uma ingasetshenziswa okwesikhashana, sicela uyigcine ngaphansi kuka-20 ° C isikhathi eside.
2. Ngemuva kokuthi ikhithi ikhishiwe futhi isetshenziswe, ikhithi kufanele igcinwe ku-4 ° C ngaphambi kokusabela ukugwema ukuqhwa okuphindaphindiwe nokuncibilika;I-Taq enzyme kufanele igcinwe ku -20°C.
3. Ngemva kokusabela, ikhithi (kuhlanganise nesilinganiso sangaphakathi sesisindo samangqamuzana kanye nesitebhisi) kufanele igcinwe "egunjini lokubona i-electrophoresis" ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ugweme ukuthintana nekhithi yokusabela kwangaphambili ukuze ugweme ukungcola.
