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I-Foregene DNA Identification System 27Y

I-Foregene DNA Identification System 27Y Isithombe Esifakiwe
  • I-Foregene DNA Identification System 27Y
  • I-Foregene DNA Identification System 27Y
  • I-Foregene DNA Identification System 27Y

Incazelo yekhithi:

I-Foregene I-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ukuze ikhulise ama-loci angu-27 Y-STR ngesikhathi esisodwa.Kufaka phakathi: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY39, DY39, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikhithi inekhono elihle lokuvimbela inhibition, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, ikhadi le-FTA, i-cotton swab, ikhadi lamathe, i-oral swab, futhi ingasetshenziswa ukukhipha nokuhlanza izifanekiso ze-DNA ezintweni zamacala.

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Incazelo

I-Foregene I-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ukuze ikhulise ama-loci angu-27 Y-STR ngesikhathi esisodwa.Kufaka phakathi: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY39, DY39, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikhithi inekhono elihle lokuvimbela inhibition, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, ikhadi le-FTA, i-cotton swab, ikhadi lamathe, i-oral swab, futhi ingasetshenziswa ukukhipha nokuhlanza izifanekiso ze-DNA ezintweni zamacala.

Okuqukethwe kwekhithi

Ikhithi yokuhlola ye-reagent

Igama lengxenye

Ivolumu (μl/ithubhu)

Ubuningi (ithubhu)

Ikhithi yokusabela kwangaphambili ye-PCR

 2×PCR Reaction Master Mix

1250

2
5 x 27Y I-Primer Mix

500

2
I-DNA Typing Standard 9948

20

2
Amanzi enziwe deionized

1700

2

Ikhithi yokusabela ye-Post-PCR

 27Y ingxube ye-alle Ladder

20

2
I-Orange yesisindo samangqamuzana angaphakathi ajwayelekile ORG500

150

2

Ukuhlaziywa Kwedatha

1. Vula isofthiwe ye-Gene Mapper ID.Lapho usebenzisa leli khithi okokuqala, udinga ukungenisa amaphaneli, isethi yokuhlaziya ehambisanayo, kanye nesilinganiso sobukhulu (i-Org500: 60, 35, 300, 30, 460, 300, 350, 450, 490, 500).

2. Ngenisa idatha ye-electrophoresis, khetha imingcele yokuhlaziya ehambisanayo njengephaneli, indlela yokuhlaziya, nezinga lesayizi, futhi ushintshe uhlobo lwesampula Lwesitebhisi lube "I-Allelic Ladder" kukholamu "Yohlobo Lwesampula";qala ukuhlaziya idatha.

I-Reagent Storage

1. Sicela uwagcine ngaphansi kokungu-20°C (uma engasetshenziswanga okwesikhashana), ngemva kokuthola amakhithi eqhwa eqhweni elomile noma amaphakethe eqhwa ejeli.

2. Sicela ugcine ikhithi ku-4°C ngaphambi kokusabela ukuze ugweme ukuqhwa okuphindaphindiwe nokuncibilika, ngemva kokuba ikhithi isikhishiwe ukuze isetshenziswe,;I-enzyme kufanele igcinwe ku-20 ° C.

2. Ngemva kokusabela, ikhithi (kuhlanganise nesilinganiso sangaphakathi sesisindo samangqamuzana kanye nesitebhisi) kufanele igcinwe "egunjini lokubona i-electrophoresis" ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ugweme ukuthintana nekhithi yokusabela kwangaphambili ukuze ugweme ukungcola.

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