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Ikhithi ye-Virus DNA/RNA (Ubuhlalu obuzibuthe)

Incazelo yekhithi:

Ubuchwepheshe obukhethekile bobuhlalu obukazibuthe buvumela ukuhlanzwa kwama-nucleic acid ekhwalithi ephezulu angenawo amaprotheni, ama-nuclease, nokunye ukungcola.

amandla angaphambili


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

I-Virus DNA/RNA Kits (Ubuhlalu Bezibuthe) amapuleti alungele ukuzisebenzela agcwaliswe kusengaphambili ngokufanelekela amasistimu okuhlanza i-Purifier HT.Ama-Nucleic acids (DNA/RNA) asuka kunkimbinkimbi enobuhlalu kazibuthe kubhafa eyakhiwe ngokukhethekile.Ubuhlalu / ama-nucleic acid complex abe esehlukaniswa nama-lysates kusetshenziswa uzibuthe.I-DNA/RNA ehlanzekile ibe isikhishwa lapho isimo sebhafa silungiswa.Ubuchwepheshe obukhethekile bobuhlalu obukazibuthe buvumela ukuhlanzwa kwama-nucleic acid ekhwalithi ephezulu angenawo amaprotheni, ama-nuclease, nokunye ukungcola.

Izingxenye zekhithi

Okuqukethwe

Amayunithi
Ipuleti 1 Isilondolozi se-MVN

500μL/kahle
Isiqephu 2 Isilondolozi se-DW1P

500μL/kahle

Isiqephu 3

 Isivimbeli i-MWP

600μL/kahle
Izinhlayiya ze-FineMag G

5μL/kahle
Isiqephu 4 I-ddH yamahhala ye-RNase2O

100μL/kahle

96 Tip Comb

1

Izici Nezinzuzo:

- Elula: ngaphandle kwe-proteinase K, ingakhishwa ngokuqondile ngokungeza u-200 μl wesampula

- Ngokushesha: Lungiselela uhlelo, ukuhlanjululwa kwesinyathelo esisodwa, ukukhipha kuthatha imizuzu eyi-12 kuphela ukuqedela ukukhishwa kwamasampula angama-96

- Ukunemba: Lungiselela i-Buffer kanye nobuhlalu obuzibuthe, ithuthukise kakhulu ukuzwela kokukhipha amagciwane, futhi yehlise kakhulu ingcuphe “yokungalungi kwamanga”

- Ukuhambisana okuhle kakhulu: ukuhambisana okuphezulu nezixazululo zesitoreji esikhuphuka nomfula kanye nama-reagents okuthola phansi komfula, ukuguquguquka okuhle, nokuzinza okuhle kakhulu kwemiphumela yokukhipha

Isicelo sekhithi

I-Virus DNA/RNA Kit (I-Magnetic Beads) yakhelwe ukuhlanzwa ngokushesha kwekhwalithi ephezulu ye-nucleic acid (i-RNA ne-DNA) egciwaneni kumasampula afana nama-swabs, amathe, igazi, i-Bodily Fluid, i-Plasma/Serum, umchamo, kanye nemidiya yezokuthutha ngegciwane (VTM).

Isitoreji kanye Nempilo yeshelufu

Wonke ama-Reagents angagcinwa ekamelweni lokushisa (15-25°C) izinyanga ezingu-12.
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