I-RT Easy II(ene-gDNase) I-Master Premix ye-cDNA yochungechunge lokuqala lwe-Real Time PCR ene-gDNase
Incazelo yekhithi:
-Ikhono elisebenzayo lokususa i-gDNA, engasusa i-gDNA kusifanekiso phakathi nemizuzu emi-2.
-Isistimu yokubhala ehlehlayo esebenza kahle, kuthatha imizuzu eyi-15 kuphela ukuqeda ukuhlanganiswa kwe-cDNA yomucu wokuqala.
-Izifanekiso eziyinkimbinkimbi: izifanekiso ezinokuqukethwe okuphezulu kwe-GC nesakhiwo sesibili esiyinkimbinkimbi nazo zingahlehliswa ngokusebenza kahle okuphezulu.
-I-High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
-I-reverse transcription system inokuqina okuphezulu kwe-thermal, izinga lokushisa elilungile lokusabela lingu-42℃, futhi isenokusebenza okuhle kokulotshiweyo okuhlehla kokungu-50℃.
Imininingwane
25×20µl rxns,100×20µl rxns
I-RT EasyTMI-II (Nge-gDNase) iwuhlelo lokuloba okuhlanekezelwe ngokukhethekile lwe-Real Time PCR ukuze kususwe ngokushesha ukungcoliswa kwe-DNA ye-genomic.I-5×gDNase Mix ingasusa ngokushesha i-genome esele ku-RNA ku-42°C imizuzu engu-2, igweme ngempumelelo ukuphazamiseka kwegenome emiphumeleni ye-qPCR.
5× RO-EasyTMImiksi iqukethe i-Foregene Reverse Transcriptase ethuthukiswe ngokukhethekile i-Foregene, okuwuhlobo olusha lwe-reverse transcriptase enobudlelwane obuqinile be-RNA.Isistimu ethuthukisiwe yenza izinga lokuloba okuhlanekezelwe lisheshe, futhi ukuhlanganiswa kwe-cDNA yomucu wokuqala kungaqedwa ngo-42°C emizuzwini eyi-15.
Isistimu yokuhlehla yokubhala yale khithi ingaphinda igcine umsebenzi omuhle ku-50°C.Ngakho-ke, kunenzuzo kakhulu kuzifanekiso ze-RNA eziyinkimbinkimbi futhi ingabhala kalula izifanekiso ze-RNA ezinokuqukethwe okuphezulu kwe-GC kanye nesakhiwo sesibili esiyinkimbinkimbi.
Izingxenye zekhithi
| 5× I-gDNase Mix |
| 5× RO-EasyTMHlanganisa |
| I-ddH yamahhala ye-RNase2O |
| Iziyalezo |
Izici&izinzuzo
-Ikhono elisebenzayo lokususa i-gDNA, engasusa i-gDNA kusifanekiso phakathi nemizuzu emi-2.
-Isistimu yokubhala ehlehlayo esebenza kahle, kuthatha imizuzu eyi-15 kuphela ukuqeda ukuhlanganiswa kwe-cDNA yomucu wokuqala.
-Izifanekiso eziyinkimbinkimbi: izifanekiso ezinokuqukethwe okuphezulu kwe-GC nesakhiwo sesibili esiyinkimbinkimbi nazo zingahlehliswa ngokusebenza kahle okuphezulu.
-I-High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
-I-reverse transcription system inokuqina okuphezulu kwe-thermal, izinga lokushisa elilungile lokusabela lingu-42℃, futhi isenokusebenza okuhle kokulotshiweyo okuhlehla kokungu-50℃.
Isicelo sekhithi
Isetshenziswa ngokuqondile ku-Real Time PCR ukuze kuhlaziywe ngokwezibalo ukusho kofuzo.
Ingakwazi ukuhlaziya ngokushesha nangokunembile ukulandelela i-RNA njengegciwane le-RNA.
Ukuhlehlisa ukulotshwa kwezifanekiso ze-RNA ezinokuqukethwe kwe-GC ephezulu noma ukwakheka kwesibili okuyinkimbinkimbi.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Ikhithi kufanele igcinwe ku -20°C.Gcina umkhiqizo esiqandisini esingaguquki sezinga lokushisa elingu-20°C ngokushesha ngemva kokuwuthola.Uma izimo zesitoreji zifanelekile, umkhiqizo ngeke wehlise noma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
