Siye saziqhenya ngokwaneliseka kwakho komthengi omkhulu nokwamukelwa okubanzi ngenxa yokuphikelela kwethu kwekhwalithi ephezulu ngokulinganayo ekuxazululeni nasekusebenzeni indlela esheshayo yokuhlukanisa nokuhlanza i-RNA ephelele kumaseli, Ngokubambelela kufilosofi yamabhizinisi amancane 'yokuqala kwekhasimende, qhubekela phambili', samukela ngobuqotho amakhasimende avela endlini yakho azosebenzisana nathi phesheya kwezilwandle.
Siye saziqhenya ngokwaneliseka kwakho kwabathengi nokwamukelwa kabanzi ngenxa yokuphikelela kwethu kwekhwalithi ephezulu ngokulinganayo kwisixazululo kanye nesevisiindlela esheshayo yokuhlukanisa kanye nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe, Inkampani yethu isebenza ngesimiso sokusebenza "sokususelwe kubuqotho, ukubambisana okudaliwe, okugxile kubantu, ukubambisana okuphumelelayo".Sithemba ukuthi singaba nobudlelwano obunobungane nosomabhizinisi abavela kuwo wonke umhlaba.

Imininingwane

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Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Siziqhenye ngokwaneliseka kwakho komthengi nokwamukelwa okubanzi ngenxa yokuphishekela kwethu ukuphishekela ikhwalithi ephezulu kokubili ngokulinganayo esixazululweni nasensizakalweni Yesikhathi Esifushane Sokuhola E-China Iminyaka Emi-2 Iwaranti Yokuhlanganisa I-Industrial Coating Ovento Ibhasi Elincane, Adheraying Industrial Coating, Adheraying Industrial Coating, Adheraying Industrial Coating of the small Bus, Adheraying Industrial Coating of the small Bus qhubekela phambili', samukela ngobuqotho amakhasimende asekhaya naphesheya kwezilwandle ukubambisana nathi.
Isikhathi Esifushane Sokuhola se-China Powder Coating Booth, I-Industrial Coating, Inkampani yethu isebenza ngesimiso sokusebenza "sokusekelwe kobuqotho, ukubambisana okudaliwe, ukusebenzelana kwabantu, ukubambisana okuphumelelayo".Sithemba ukuthi singaba nobudlelwano obunobungane nosomabhizinisi abavela kuwo wonke umhlaba.