Umsebenzi wethu kufanele kube ukuhlinzeka abasebenzisi bethu bokugcina kanye nabathengi ngekhwalithi ephezulu kakhulu nemikhiqizo yedijithali ephathekayo eqhudelanayo nezisombululoI-PCR Reagent, Samukela ngobuqotho abahlanganyeli bamabhizinisi aphesheya kwezilwandle nasekhaya, futhi sithemba ukusebenza nawe esikhathini eside!
Umsebenzi wethu kufanele kube ukuhlinzeka abasebenzisi bethu bokugcina kanye nabathengi ngekhwalithi ephezulu kakhulu nemikhiqizo yedijithali ephathekayo eqhudelanayo nezisombululoI-PCR Reagent, Njengoba sinesipiliyoni seminyaka ecishe ibe ngu-30 kwezamabhizinisi, besiqiniseka ngenkonzo ephakeme, ikhwalithi nokulethwa.Samukela ngemfudumalo amakhasimende avela emhlabeni wonke ukuze abambisane nenkampani yethu ukuze kuthuthukiswe okufanayo.

Imininingwane

5ml, 10ml, 40ml

Izingxenye zekhithi

Izici&izinzuzo

■ Ukwethembeka okuphezulu: izikhathi eziyisi-6 kune-enzyme ye-Taq evamile;

■ Isivinini sokukhulisa esisheshayo : ijubane lokukhulisa ngu-5-10sec/kb, eliphindwe izikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;

■ Ukuguquguquka okwengeziwe kwesifanekiso: Ingakwazi ukukhulisa izifanekiso ze-DNA eziyinkimbinkimbi ngokuphezuluOkuqukethwe kwe-GC;

■ Ukusebenza okuphakeme kokukhulisa amandla: imijikelezo yokukhulisa ephansi kune-enzyme ye-Taq evamile;

∎ Ukubekezelelana kwemvelo kunamandla: ibekwe ku-37°C isonto lonke, igcina umsebenzi ongaphezu kwama-90%;

■ Inomsebenzi ongu-5′ →3′ we-DNA polymerase kanye no-5′→3′ umsebenzi we-exonuclease, ngaphandle komsebenzi we-3′ →5′ we-exonuclease.

■ Ikhithi ixutshwe ngaphambili ne-Electrophoresis Loading Buffer, ngemva kokuba i-PCR isiqediwe, isampula ingalayishwa ngokuqondile i-electrophoresis;ukusebenza kulula futhi kulula.

Isicelo sekhithi

I-PCR yokukhulisa izingcezu ze-DNA, ukulebula i-DNA, ukulandelana kwe-DNA,I-PCR kanye nomsila we-A

Isitoreji kanye Nempilo yeshelufu

Ingagcinwa isikhathi eside ku -20°C;ukusetshenziswa njalo, ingagcinwa ku-4°C isikhathi esifushane.I-2×Real PCR EasyTM Mix-SYBR ehlinzekwa yi-Real Time PCR EasyTM-SYBR Green I kit iyisistimu entsha ye-premix esebenzisa i-SYBR Green I Yesikhathi Sangempela ukusabela kokukhulisa i-PCR, okungathuthukisa kakhulu ukucaciswa komkhiqizo nokuzwela kokusabela.I-ROX inikezwa njengodayi wokulawula wangaphakathi.Amandla e-fluorescence ale khithi aphindwe izikhathi ezingu-3-5 kunemikhiqizo efanayo, engazwela kakhulu futhi ibonise ngokunembile ukugxila kwesifanekiso se-DNA esihlosiwe.
I-2×Real PCR EasyTM Mix-SYBR iqukethe i-Taq DNA Polymerase eyingqayizivele ye-Foregene.Uma iqhathaniswa nama-enzyme e-Taq ajwayelekile, inezinzuzo zokusebenza kahle kokukhulisa i-amplification, ikhono elithile eliqinile lokukhulisa kanye nezinga lokungafani eliphansi.Inganciphisa ukukhulisa okungaqondile futhi ithuthukise ukunemba kwe-PCR.