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Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT

Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT Isithombe Esifakiwe
  • Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT
  • Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT
  • Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT
  • Kulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT

Incazelo yekhithi:

Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi;ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula eyingqayizivele.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ehlanziwe ingonakalisiwe;i-Buffer RW1, i-Buffer RW2 buffer system yokugeza iqinisekisa i-RNA etholiwe engenawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwezinto eziphilayo.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sizibophezele ekunikezeni isevisi yokuthenga yabathengi elula, eyonga isikhathi kanye neyonga imaliKulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT, Samukela ngemfudumalo osomabhizinisi abancane abavela kuzo zonke izizinda zokuphila, sithemba ukusungula ibhizinisi elinobungane nelinokubambisana ukuze baxhumane nawe futhi bazuze umgomo wokuwina.
Sizibophezele ekunikezeni isevisi yokuthenga yabathengi elula, eyonga isikhathi kanye neyonga imaliKulula ukusebenzisa i-CELL TOTAL RNA ISOLATION KIT, manje sesibheke phambili ekubambisaneni okukhulu nakakhulu namakhasimende aphesheya okusekelwe ezinzuzweni ezifanayo.Sizosebenza ngenhliziyo yonke ukuthuthukisa okuthengiswayo kwethu namasevisi.Siphinde sithembise ukusebenza ngokubambisana nozakwethu bebhizinisi ukuze sikhuphulele ukubambisana kwethu ezingeni eliphezulu futhi sabelane ngempumelelo ndawonye.Siyakwamukela ngokufudumele ukuthi uvakashele imboni yethu ngobuqotho.

Imininingwane

50 Preps, 200 Preps

Izingxenye zekhithi

I-Buffer cRL1, i-Buffer cRL2

Isilondolozi RW1, Isilondolozi RW2

I-ddH yamahhala ye-RNase2O, Ikholomu Yokuhlanza I-DNA

Ikholomu Ye-RNA Kuphela

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

isamba seseli RNA

Umdwebo

Ukugeleza Komsebenzi Wekhithi Yokuzihlukanisa Yeseli ye-RNA1

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela).Sizibophezele ekunikezeni insizakalo yokuthenga elula, eyonga isikhathi kanye neyonga imali eyodwa-stop yomthengi ukuze athengise Okushisayo I-China Robenidine Hydrochloride Premix Best Selling Feed Ingredient, Sikwenza ngokufudumele ukuxhumana nebhizinisi elincane ukuya ebhizinisini elincane. futhi uzuze inhloso yokuwina-win.
Ukuthengisa okushisayo Isengezo Sokuphakelayo sase-China, Ibanga Lokuphakelayo, manje sesibheke ngabomvu ukubambisana okukhulu nakakhulu namakhasimende aphesheya okusekelwe ezinzuzweni ezifanayo.Sizosebenza ngenhliziyo yonke ukuthuthukisa okuthengiswayo kwethu namasevisi.Siphinde sithembise ukusebenza ngokubambisana nozakwethu bebhizinisi ukuze sikhuphulele ukubambisana kwethu ezingeni eliphezulu futhi sabelane ngempumelelo ndawonye.Siyakwamukela ngokufudumele ukuthi uvakashele imboni yethu ngobuqotho.

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