Sisebenzisa uhlelo oluphelele lwesayensi lokuphatha lwekhwalithi enhle, ikhwalithi enhle kakhulu nokholo oluphakeme, sazuza ukuma okuhle futhi sahlala kulesi simiso ngentengo ephansi yasefekthri I-China Long Taq DNA Polymerase, i-Long PCR, Samukela ngobuqotho zonke izivakashi ukuze zihlele ubudlelwano benkampani nathi ngesisekelo sezici ezinhle ezifanayo.Kufanele uxhumane nathi manje.Ungathola impendulo yethu enekhono phakathi namahora angu-8 ambalwa.
Sisebenzisa uhlelo olugcwele lwesayensi lwekhwalithi enhle, ikhwalithi enhle kakhulu nokholo oluphakeme, sizuza ukuma okuhle futhi sathatha lesi siqondisoI-China PCR Amplification, Qpcr, Inkampani yethu ihlanganisa indawo 20, 000 square metres.Sinezisebenzi ezingaphezu kuka-200, ithimba lochwepheshe lochwepheshe, isipiliyoni seminyaka engu-15, ubuciko obuhle kakhulu, izinga elizinzile nelithembekile, intengo yokuncintisana namandla okukhiqiza anele, le yindlela esenza ngayo amakhasimende ethu aqine.Uma unombuzo, sicela ungangabazi ukusithinta.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2 × PCR EasyTM Mix, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku ezingu-10). Sisebenzisa uhlelo oluphelele lwesayensi lokuphatha lwekhwalithi enhle, ikhwalithi enhle kakhulu nokholo oluphakeme, sizuza ukuma okuhle futhi sathatha lesi siyalo ngentengo ephansi yefekthri I-China Long Taq DNA Polymerase, I-PCR ende, Sihlela ngobuqotho ubudlelwano obuhle benkampani nezivakashi.Kufanele uxhumane nathi manje.Ungathola impendulo yethu enekhono phakathi namahora angu-8 ambalwa.
intengo ephansi yefekthriI-China PCR Amplification, Qpcr, Inkampani yethu ihlanganisa indawo 20, 000 square metres.Sinezisebenzi ezingaphezu kuka-200, ithimba lochwepheshe lochwepheshe, isipiliyoni seminyaka engu-15, ubuciko obuhle kakhulu, izinga elizinzile nelithembekile, intengo yokuncintisana namandla okukhiqiza anele, le yindlela esenza ngayo amakhasimende ethu aqine.Uma unombuzo, sicela ungangabazi ukusithinta.