Ngesikweletu sebhizinisi esiphusile, isevisi enhle kakhulu yangemva kokuthengisa kanye nezindawo zokukhiqiza zesimanje, sizuze idumela elihle kakhulu kumakhasimende ethu emhlabeni jikeleleisethi ephelele yama-reagents, Umgomo wefemu yethu kufanele kube ukuhlinzeka ngezimpahla zekhwalithi ephezulu ezinamanani angcono kakhulu.Besikubheke ngabomvu ukwenza inhlangano nawe!
Ngesikweletu sebhizinisi esiphusile, isevisi enhle kakhulu yangemva kokuthengisa kanye nezindawo zokukhiqiza zesimanje, sizuze idumela elihle kakhulu kumakhasimende ethu emhlabeni jikeleleisethi ephelele yama-reagents, Ngokusekelwe ezimpahleni ezinekhwalithi ephezulu, intengo yokuncintisana, kanye nenkonzo yethu egcwele uhla oluphelele, manje sesiqongelele amandla afanelekayo nolwazi, futhi sizakhele idumela elihle kakhulu ensimini.Kanye nentuthuko eqhubekayo, asizibophezeli ebhizinisini lasekhaya laseShayina kuphela kodwa nasezimakethe zomhlaba wonke.Kwangathi ungashukunyiswa izinto zethu zekhwalithi ephezulu kanye nenkonzo enothando.Masivule isahluko esisha senzuzo efanayo futhi siwine kabili.

Imininingwane

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Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela). Ngesikweletu sebhizinisi esizwakalayo, isevisi enhle kakhulu yangemva kokuthengisa kanye nezindawo zokukhiqiza zesimanje, sizuze idumela elihle kakhulu kumakhasimende ethu emhlabeni wonke ngeFactory ngqo I-China Chemical Reagent Bloom Tech Citric Acid CAS 97-92 yethu engcono kakhulu kufanele ihlinzeke ngezintengo ezinhle kakhulu zekhwalithi ephezulu. g.Besikubheke ngabomvu ukwenza inhlangano nawe!
Ifekthri iqonde ngqo i-China Chemical Reagent, i-Research Chemical, Isekelwe ezimpahleni ezinekhwalithi ephezulu, intengo yokuncintisana, kanye nenkonzo yethu egcwele uhla oluphelele, manje sesiqongelele amandla afanelekayo nolwazi, futhi sesizakhele idumela elihle kakhulu kulo mkhakha.Kanye nentuthuko eqhubekayo, asizibophezeli ebhizinisini lasekhaya laseShayina kuphela kodwa nasezimakethe zomhlaba wonke.Kwangathi ungashukunyiswa izinto zethu zekhwalithi ephezulu kanye nenkonzo enothando.Masivule isahluko esisha senzuzo efanayo futhi siwine kabili.