Imbewu Yezitshalo(Encane Naphakathi) I-PCR Kit I-UNG eqondile (ngaphandle kwamathuluzi okusampula)
Izincazelo
Lo mkhiqizo usebenzisa isistimu yokusabela ye-lysis eyingqayizivele, engakhulula ngokushesha i-DNA ye-genomic kumasampula embewu yezitshalo enokuqukethwe okuphansi kwe-polysaccharide kanye ne-polyphenol (njengalezi: irayisi, ukolweni, ugwayi, ummbila, ubhontshisi wesoya, njll.) ukuze isetshenziswe ekuphenduleni kwe-PCR, ngakho-ke ifaneleka ngokukhethekile ukuhlolwa kofuzo kwesilinganiso esikhulu.Ukuze kuhlangatshezwane nezidingo zokuhlolwa kokutholwa okuhlukile, le kit ingasebenzisa izinhlobo eziningi zamasampuli (njengembewu ephelele, ukusikeka kwezicubu ezincane, noma amasampula aphansi embewu eminingi) ukuze kuhlolwe.Phakathi kwazo, kumasampula embewu asadinga ukuhluma, ungakhetha ukusika ukusikeka kwezicubu ezincane (1-5mg) ngaphandle kombungu wembewu ukuze uhlolwe;ngokuhlolwa okudingeka kuthathwe enanini elikhulu lamasampuli, ungakhetha ukuhlanganisa amasampuli amaningi futhi uwahlanganise.Ngemva kokugaya zibe izinhlayiya ezinobubanzi obungaba ngu-0.5mm, ukuhlolwa kungenziwa (okungenani u-0.1% wesampula okuhlosiwe kungatholwa).
Imininingwane
50T,200T,500T,2000T
Izingxenye zekhithi
Plant Seed Direct PCR Kit I-UNG Imbewu Yezitshalo (Encane Naphakathi) I-PCR Kit-UNG eqondile | |||||
Ukwakheka kwekhithi | I-TP-0312T | I-TP-03121 | I-TP-03122 | TP-03123 | |
50 T | 200 T | 500 T | 2000 T | ||
Ingxenye I | Isilondolozi SP1 | 10ml | 40ml | 100ml | 400ml |
Isilondolozi se-SP2 | 3ml | 10ml | 25ml | 100ml | |
I-6× I-DNA Yokulayisha Ibhafa | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Ingxenye II | 2× Imbewu PCR KululaTMImiksi(UNG) | 500μl | 1mlx2 | 1.7ml×3 | 1.7ml×12 |
Imanuwali | 1 | 1 | 1 | 1 |
Izici nezinzuzo
- Asikho isidingo esithatha isikhathi esiningi futhi esibizayo ukuhlanzwa kwe-DNA kuyadingeka.
- Isidingo sesampula sincane, thatha nje imbewu eyodwa.
- Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukusebenza kulula.
- Uhlelo lwe-PCR oluthuthukisiwe lwenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.
- Isistimu ye-PCR yokulwa nokungcoliswa 2× Imbewu PCR KululaTMI-Mix (UNG) ingaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukucaciswa nokunemba kokukhulisa.
Ukuhamba komsebenzi
Izimo zokugcina
1.Ezokuthutha
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi Ingxenye II isesimweni esingu-<4 ℃.
2.Isitoreji
Ingxenye I yale kit kufanele igcinwe ekamelweni lokushisa noma 2-8 ° C.
Ingxenye II kufanele igcinwe ku -20°C.