“Ikhwalithi yokuqala, Ukwethembeka njengesisekelo, Usizo oluqotho kanye nenzuzo efanayo” kuwumbono wethu, njengendlela yokukhiqiza ngokuqhubekayo futhi siphishekele ubuhle Bentengo Yezitolo I-China Cost-Effective Manufacturer Wholesales I-Universal DNA Rna Isolation Kit Reagents, Ethonywe kusukela endaweni yemakethe yokwakha esheshayo yokudla okusheshayo nesiphuzo okwenza kube nemiphumela emihle kakhulu yezimpahla ezithengwayo/esiphuzweni ukuze siqhubekisele phambili izinto ezithengwayo/ezisetshenziswayo. ndawonye.
"Ikhwalithi yokuqala, Ukwethembeka njengesisekelo, usizo oluqotho kanye nenzuzo efanayo" umqondo wethu, njengendlela yokukhiqiza ngokuqhubekayo nokuphishekela ubuhleUkuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, I-China Universal Rna DNA Reagents, Inkampani yethu manje ineminyango eminingi, futhi kunezisebenzi ezingaphezu kuka-20 enkampanini yethu.Sakha isitolo sokuthengisa, igumbi lokubonisa, kanye nesitolo somkhiqizo.Okwamanje, sabhalisa ibhrendi yethu.Siqinise ukuhlolwa kwekhwalithi yomkhiqizo.

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela).”Ikhwalithi yokuqala, Ukwethembeka njengesisekelo, usizo oluqotho kanye nenzuzo efanayo” kuwumbono wethu, njengendlela yokukhiqiza ngokuqhubekayo nokuphishekela ubuhle be-Wholesale Price China Cost-Effective Manufacturer Manufacturer I-Universal Manufacturer I-Redfluence Manufacturer Imakethe ye-Redfluence Manufacturer DNA yokudla okusheshayo neziphuzo ezisetshenziswayo emhlabeni wonke , Sifuna phambili ekusebenzeni nozakwethu/amaklayenti ukuze senze imiphumela emihle sindawonye.
I-Wholesale PriceI-China Universal Rna DNA Reagents, Ukuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, Inkampani yethu manje ineminyango eminingi, futhi kunezisebenzi ezingaphezu kuka-20 enkampanini yethu.Sakha isitolo sokuthengisa, igumbi lokubonisa, kanye nesitolo somkhiqizo.Okwamanje, sabhalisa ibhrendi yethu.Siqinise ukuhlolwa kwekhwalithi yomkhiqizo.