Sineqembu elisebenza kahle ngempela elizobhekana nemibuzo evela kwabahlomulayo.Inhloso yethu “ukugcwalisa amakhasimende angu-100% ngomkhiqizo wethu omuhle kakhulu, intengo kanye nesevisi yeqembu” futhi sijabulela irekhodi elihle kakhulu phakathi kwamakhasimende.Ngamafekthri amaningi, singaletha kalula ukukhetha okubanzi kwe-China Intengo Eshibhile i-China 100t/Kit Extraction-Free Nucleic Acid Release Reagent ye-Genetic PCR Detection, Amalungu eqembu lethu anomgomo wokuhlinzeka okuthengiswayo ngesilinganiso esibalulekile sezindleko zokusebenza kubathengi bethu, futhi okuhlosiwe ngathi sonke ngokuvamile ukwanelisa abathengi bethu abavela kuyo yonke indawo ezungezile.
Sineqembu elisebenza kahle ngempela elizobhekana nemibuzo evela kwabahlomulayo.Inhloso yethu “ukugcwalisa amakhasimende angu-100% ngomkhiqizo wethu omuhle kakhulu, intengo kanye nesevisi yeqembu” futhi sijabulela irekhodi elihle kakhulu phakathi kwamakhasimende.Ngamafekthri amaningi, singaletha kalula ukukhetha okubanziI-China DNA Rna Release Reagent, I-DNA Release Reagent, Qiniseka ukuthi uzizwa ukhululekile ngempela ukusithumela izidingo zakho futhi sizokuphendula ngokushesha.Manje sineqembu lobunjiniyela elinekhono elizokusebenzela cishe zonke izidingo ezinemininingwane.Amasampula amahhala angathunyelwa ukuze afanele izidingo zakho mathupha ukuze kuqondwe ulwazi olwengeziwe.Ngomzamo wokuhlangabezana nezidingo zakho, qiniseka ukuthi uzizwa ukhululekile ukuxhumana nathi.Ungasithumela ama-imeyili futhi usithinte ngokuqondile.Ngaphezu kwalokho, siyakwamukela ukuvakashelwa efekthri yethu evela emhlabeni wonke ukuze kuqashelwe kangcono inhlangano yethu.nd izinto.Ekuhwebeni kwethu nabathengisi bamazwe amaningi, sivamise ukunamathela kumgomo wokulingana nokuhlomula ngakho konke.Kuyithemba lethu ngempela ukumaketha, ngemizamo ehlanganyelwe, ukuhwebelana ngakunye nobungane ukuze kuzuze thina sobabili.Sibheke ngabomvu ukuthola imibuzo yakho.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo

∎ Isidingo sesampula sincane, njengoba izicubu zezilwane ezingama-5mg zingahlolwa.

■ Akukho ukwelashwa okukhethekile okufana nokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

■ Uhlelo lwe-PCR olulwa nokungcola 2×PCR Lulula^TMI-Mix (UNG) iqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekisa ukucaciswa nokunemba kokukhulisa.

Imingcele yekhithi

Isicelo: izicubu ezihlukahlukene zezilwane.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer AL ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2 × PCR Easy^TMI-Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi nezinsuku eziyi-10).Sineqembu elisebenza kahle ngempela ukubhekana nemibuzo evela kwabangase balindele.Inhloso yethu “ukugcwalisa amakhasimende angu-100% ngomkhiqizo wethu omuhle kakhulu, intengo kanye nesevisi yeqembu” futhi sijabulela irekhodi elihle kakhulu phakathi kwamakhasimende.Ngamafekthri amaningi, singaletha kalula ukukhetha okubanzi kwe-China Intengo Eshibhile i-China 100t/Kit Extraction-Free Nucleic Acid Release Reagent ye-Genetic PCR Detection, Amalungu eqembu lethu anomgomo wokuhlinzeka okuthengiswayo ngesilinganiso esibalulekile sezindleko zokusebenza kubathengi bethu, futhi okuhlosiwe ngathi sonke ngokuvamile ukwanelisa abathengi bethu abavela kuyo yonke indawo ezungezile.
China Intengo eshibhileI-China DNA Rna Release Reagent, I-DNA Release Reagent, Qiniseka ukuthi uzizwa ukhululekile ngempela ukusithumela izidingo zakho futhi sizokuphendula ngokushesha.Manje sineqembu lobunjiniyela elinekhono elizokusebenzela cishe zonke izidingo ezinemininingwane.Amasampula amahhala angathunyelwa ukuze afanele izidingo zakho mathupha ukuze kuqondwe ulwazi olwengeziwe.Ngomzamo wokuhlangabezana nezidingo zakho, qiniseka ukuthi uzizwa ukhululekile ukuxhumana nathi.Ungasithumela ama-imeyili futhi usithinte ngokuqondile.Ngaphezu kwalokho, siyakwamukela ukuvakashelwa efekthri yethu evela emhlabeni wonke ukuze kuqashelwe kangcono inhlangano yethu.nd izinto.Ekuhwebeni kwethu nabathengisi bamazwe amaningi, sivamise ukunamathela kumgomo wokulingana nokuhlomula ngakho konke.Kuyithemba lethu ngempela ukumaketha, ngemizamo ehlanganyelwe, ukuhwebelana ngakunye nobungane ukuze kuzuze thina sobabili.Sibheke ngabomvu ukuthola imibuzo yakho.