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I-Animal Total RNA Isolate Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA ephelele nekhwalithi ephezulu kuzicubu zezilwane ezahlukahlukene. Inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kwi-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Uhlelo lonke alunayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer RW1 neBuffer RW2 zingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.
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Iseli Ingqikithi ye-RNA Isolate Kit
Le khithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA esezingeni eliphezulu nekhwalithi ephezulu kumaseli akhuliswe kuma-96, 24, 12, kanye nama-6-well plate. Ikhithi inikeza i-DNA-Cleaning Column ephumelelayo, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA. Ukusebenza kulula futhi konga isikhathi; Ikholomu ye-RNA kuphela ingabopha i-RNA kahle ngefomula elihlukile. Inani elikhulu lamasampuli lingacutshungulwa ngasikhathi sinye.
Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ehlanziwe ingehliswa; iBuffer RW1, iBuffer RW2 buffer washing system ibeka i-RNA etholakalayo ngaphandle kwamaprotheni, i-DNA, i-ion, kanye nokungcola kwe-organic compound.
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Igciwane le-Viral DNA RNA Isolate
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle ubumsulwa bekhwalithi ephezulu nekhwalithi ephezulu ye-DNA ne-RNA kusuka kumasampula anjenge-plasma, i-serum, uketshezi lomzimba olungenamaseli, kanye namandla angaphezu kwamasiko. Ikhithi ingeza ngqo i-Linear Acrylamide, engabamba kalula inani elincane le-DNA ne-RNA kusuka kumasampula. Ikholomu ye-DNA / RNA-kuphela engabopha kahle i-DNA ne-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Ikhithi yonke ayiqukethe i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer RW1 neBuffer RW2 ingaqinisekisa ukuthi i-viral nucleic acid etholakala ngaphandle kwamaprotheni, i-nuclease noma okunye ukungcola, okungasetshenziswa ngqo ekuhlolweni kwe-biology yamangqamuzana esezansi.
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Isitshalo Esiphelele se-RNA Isolate Plus Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA esezingeni eliphezulu nekhwalithi ephezulu kuzicubu zezitshalo ezahlukahlukene ezinokuqukethwe okuphezulu kwe-polysaccharides noma okuqukethwe kwe-polyphenols. Inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kwi-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Ikhithi yonke ayiqukethe i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer PRW1 neBuffer PRW2 ingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.
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I-Viral DNA ne-RNA Isolate Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle ubumsulwa bekhwalithi ephezulu nekhwalithi ephezulu ye-DNA ne-RNA kusuka kumasampula anjenge-plasma, i-serum, uketshezi lomzimba olungenamaseli, kanye namandla angaphezu kwamasiko. Ikhithi ingeza ngqo i-Linear Acrylamide, engabamba kalula inani elincane le-DNA ne-RNA kusuka kumasampula. Ikholomu ye-DNA / RNA-kuphela engabopha kahle i-DNA ne-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Ikhithi yonke ayiqukethe i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer RW1 neBuffer RW2 ingaqinisekisa ukuthi i-viral nucleic acid etholakala ngaphandle kwamaprotheni, i-nuclease noma okunye ukungcola, okungasetshenziswa ngqo ekuhlolweni kwe-biology yamangqamuzana esezansi.
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Igciwane le-RNA Isolate Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA yegciwane eliphakeme nekhwalithi ephezulu kumasampuli anjenge-plasma, i-serum, uketshezi lomzimba olungenamaseli, kanye namandla angaphezu kwamasiko weseli. Ikhithi ingeza ngqo i-Linear Acrylamide, engabamba kalula inani elincane le-RNA kumasampuli. Ikholomu ye-RNA-kuphela engabopha kahle i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Ikhithi yonke ayiqukethe i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. I-Buffer viRW1 ne-Buffer viRW2 ingaqinisekisa ukuthi i-virus nucleic acid etholakalayo ayinayo amaprotheni, i-nuclease noma okunye ukungcola, okungasetshenziswa ngqo ekuhlolweni kwe-biology yamangqamuzana esezansi.
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Isitshalo Esiphelele se-RNA Isolate Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA ephezulu kakhulu nekhwalithi ephezulu kuzicubu zezitshalo ezahlukahlukene ezinokuqukethwe okuphansi kwe-polysaccharides kanye ne-polyphenols. Ngamasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwe-polyphenols, kunconywa ukuthi kusetshenziswe iPlant Total RNA Isolate Plus Kit ukuthola imiphumela engcono yokukhipha i-RNA. Ikhithi inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kusuka ku-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Uhlelo lonke alunayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer PRW1 neBuffer PRW2 ingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.
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Isitshalo Esiphelele seRNA Isolate Kit Plus
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA esezingeni eliphezulu nekhwalithi ephezulu kuzicubu zezitshalo ezahlukahlukene ezinokuqukethwe okuphezulu kwe-polysaccharides noma okuqukethwe kwe-polyphenols. Inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kwi-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Ikhithi yonke ayiqukethe i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer PRW1 neBuffer PRW2 ingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.
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Isitshalo se-DNA Isolate
Leli thuluzi lisebenzisa ikholomu ye-DNA kuphela ekwazi ukubopha ngqo i-DNA, i-Foregene protease nohlelo oluyingqayizivele lwe-buffer, olwenza kube lula ukuhlanzwa kwe-DNA genomic yezitshalo. Ikhwalithi ephezulu ye-genomic DNA ingatholwa kungakapheli imizuzu engama-30, evikela ukwehliswa kwe-genomic DNA.
Ulwelwesi lwe-silica gel ulwelwesi olusetshenziswa kuphela kukholamu ye-spin yizinto ezintsha ezihlukile ze-Foregene, ezingabopha ngempumelelo nangokuqondile i-DNA, futhi zikhulise ukususwa kwe-RNA, amaprotheni okungcola, ama-ion, ama-polysaccharides, ama-polyphenols namanye ama-organic compounds.
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I-General Plasmid Mini Kit
Lo mkhiqizo usebenzisa ubuchwepheshe obuyingqayizivele bekholomu yokuhlanzwa kwe-DNA nefomula ye-SDS esebenza kahle, engathola i-DNA yekhwalithi ephezulu kuma-bacteria kungakapheli imizuzu engama-20.
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Izicubu ze-DNA Tissue Kit
Leli thuluzi lisebenzisa ikholomu ye-DNA kuphela ekwazi ukubopha ngqo i-DNA, i-Foregene protease nohlelo oluyingqayizivele lwe-buffer. Ikhwalithi ephezulu ye-genomic DNA ingakhishwa kumaseli ahlukahlukene akhulisiwe nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengu-50.
Ulwelwesi lwe-silica gel ulwelwesi olusetshenziswa kuphela kukholamu ye-spin luyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokuqondile i-DNA, futhi ikhulise ukususwa kwe-RNA, amaprotheni okungcola, ama-ion namanye amakhompiyutha wemvelo kumaseli. I-5-80μg esezingeni eliphezulu ye-genomic DNA ingahlanzwa kusuka kuzicubu ze-10-50mg.
