Ukuphishekela kwethu kanye nenhloso yenhlangano ukuthi “Njalo sanelise izidingo zamakhasimende ethu”.Siyaqhubeka nokuthuthukisa kanye nesitayela izinto ezimangalisayo zekhwalithi ephezulu kumthengi wethu ngamunye osephelelwe yisikhathi nabasha futhi sifeza ithemba lokuwina kumakhasimende ethu njengathi eFactory For China.I-Nucleic Acid Isolation& Ikhithi Yokuhlanza (I-MagPure Universal RNA Kit), Umgomo webhizinisi lethu ukwethula izinto zekhwalithi ephezulu, ukwesekwa kochwepheshe, nokuxhumana okuyiqiniso.Yamukela bonke abangani abaseduze ukuze bazame ukuthenga ubudlelwano bebhizinisi besikhathi eside.
Ukuphishekela kwethu kanye nenhloso yenhlangano ukuthi “Njalo sanelise izidingo zamakhasimende ethu”.Siyaqhubeka nokuthuthukisa kanye nesitayela izinto ezimangalisayo zekhwalithi ephezulu kumthengi wethu ngamunye osephelelwe yisikhathi nabasha futhi sifeza ithuba lokuwina-wina kumakhasimende ethu ngendlela efanayo njengathiChina Rna/DNA Isizinda Ukuhlanzwa, I-Nucleic Acid Isolation, Imfundiso yethu “iwubuqotho kuqala, ikhwalithi engcono kakhulu”.Siyazethemba ekukunikezeni isevisi enhle kakhulu nemikhiqizo ekahle.Sithemba ngobuqotho ukuthi singasungula ukubambisana kwebhizinisi lokuwina-win nawe esikhathini esizayo!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Injongo yethu yokuphishekela kanye nenhlangano iwukuba “Ukwanelisa njalo izidingo zeklayenti lethu”.Siyaqhubeka nokuthuthukisa kanye nesitayela izinto ezimangalisayo zekhwalithi ephezulu kumthengi wethu ngamunye osephelelwe yisikhathi nabasha futhi sifeza ithemba lokuwina kumakhasimende ethu njengathi eFactory For China.I-Nucleic Acid Isolation& Ikhithi Yokuhlanza (I-MagPure Universal RNA Kit), Umgomo webhizinisi lethu ukwethula izinto zekhwalithi ephezulu, ukwesekwa kochwepheshe, nokuxhumana okuyiqiniso.Yamukela bonke abangani abaseduze ukuze bazame ukuthenga ubudlelwano bebhizinisi besikhathi eside.
Factory ForChina Rna/DNA Isizinda Ukuhlanzwa, I-Nucleic Acid Isolation, Inkolelo yethu “iwubuqotho kuqala, ikhwalithi engcono kakhulu”.Siyazethemba ekukunikezeni isevisi enhle kakhulu nemikhiqizo ekahle.Sithemba ngobuqotho ukuthi singasungula ukubambisana kwebhizinisi lokuwina-win nawe esikhathini esizayo!