Ngokuphatha kwethu okuhle kakhulu, amandla obuchwepheshe aqinile kanye nendlela eqinile yokulawula enhle kakhulu, siyaqhubeka nokunikeza amakhasimende ethu ikhwalithi enhle enomthwalo wemfanelo, izindleko ezifanele kanye nezinkampani ezinhle.Sihlose ukubhekwa njengozakwethu abanomthwalo wemfanelo omkhulu futhi sizuze injabulo yakho nge-Supply OEM Virus DNA kanye ne-Rna Isolation Extraction Kit, Sibheke ngabomvu ukusungula ubudlelwano obuhle bokubambisana namakhasimende asekhaya nakwamanye amazwe ukuze sakhe ikusasa eliqhakazile ndawonye.
Ngokuphatha kwethu okuhle kakhulu, amandla obuchwepheshe aqinile kanye nendlela eqinile yokulawula enhle kakhulu, siyaqhubeka nokunikeza amakhasimende ethu ikhwalithi enhle enomthwalo wemfanelo, izindleko ezifanele kanye nezinkampani ezinhle.Sihlose ukuthi sithathwe njengomunye wozakwethu abanomthwalo wemfanelo futhi sizuzele injabulo yakhoI-China Nucleic Acid Reagent kanye ne-DNA/Rna Extraction Kit, Manje sinesipiliyoni seminyaka engaphezu kwe-10 sokukhiqiza kanye nebhizinisi lokuthekelisa.Sihlala sithuthukisa futhi siklama izinhlobo ezintsha zokuthengiswayo ukuze zihlangabezane nesidingo semakethe futhi sisize izivakashi ngokuqhubekayo ngokubuyekeza imikhiqizo yethu.Sibe ngumkhiqizi okhethekile kanye nesithekeli e-China.Noma ngabe ukuphi, qiniseka ukuthi uyasijoyina, futhi ngokubambisana sizokwakha ikusasa eliqhakazile emkhakheni wakho webhizinisi!
Amamanuwali wemiyalo:

Ngokuphatha kwethu okuhle kakhulu, amandla obuchwepheshe aqinile kanye nendlela eqinile yokulawula enhle kakhulu, siyaqhubeka nokunikeza amakhasimende ethu ikhwalithi enhle enomthwalo wemfanelo, izindleko ezifanele kanye nezinkampani ezinhle.Sihlose ukubhekwa njengozakwethu abanomthwalo wemfanelo omkhulu futhi sizuze injabulo yakho nge-Supply OEM Virus DNA kanye ne-Rna Isolation Extraction Kit, Sibheke ngabomvu ukusungula ubudlelwano obuhle bokubambisana namakhasimende asekhaya nakwamanye amazwe ukuze sakhe ikusasa eliqhakazile ndawonye.
Faka i-OEMI-China Nucleic Acid Reagent kanye ne-DNA/Rna Extraction Kit, Manje sinesipiliyoni seminyaka engaphezu kwe-10 sokukhiqiza kanye nebhizinisi lokuthekelisa.Sihlala sithuthukisa futhi siklama izinhlobo ezintsha zokuthengiswayo ukuze zihlangabezane nesidingo semakethe futhi sisize izivakashi ngokuqhubekayo ngokubuyekeza imikhiqizo yethu.Sibe ngumkhiqizi okhethekile kanye nesithekeli e-China.Noma ngabe ukuphi, qiniseka ukuthi uyasijoyina, futhi ngokubambisana sizokwakha ikusasa eliqhakazile emkhakheni wakho webhizinisi!

Umhlahlandlela Wokuhlaziya Inkinga

Okulandelayo ukuhlaziya izinkinga ezingase kuhlangatshezwane nazo ekukhishweni kwe-viral DNA/RNA, ngethemba lokuba usizo ekuhloleni kwakho.Ngaphezu kwalokho, kwezinye izinkinga zokuhlola noma zobuchwepheshe ngaphandle kwemiyalo yokusebenza nokuhlaziya inkinga, sinikele ngosekelo lobuchwepheshe ukuze sikusize.Uma unezinye izidingo, sicela usithinte: 028-83360257 noma E-mail:

Tech@foregene.com.

Akukho ukukhishwa kwe-nucleic acid noma isivuno esiphansi se-nucleic acid

Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-nucleic acid, indlela yokusebenza, ivolumu ye-elution, njll.

Ukuhlaziywa kwezimbangela ezijwayelekile:

1. Ukugeza kweqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation yenziwa ngesikhathi senqubo.

Ukusikisela: Sebenzisa ekamelweni lokushisa (15-25°C) kuyo yonke inqubo, ungabhavi eqhweni kanye nokushisa okuphansi kwe-centrifugation.

2. Isampula ligcinwe ngendlela engafanele noma isampula ligcinwe isikhathi eside kakhulu.

Okutuswayo: Gcina amasampula ku -80°C futhi ugweme ukuqhwa okuphindaphindiwe nokuncibilika;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-nucleic acid.

3. Isampula yesampula enganele.

Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesisombululo sokusebenza se-lysis kuxutshwe kahle futhi kufakwe endaweni yokushisa yegumbi (15-25°C) imizuzu eyi-10.

4. Ukwengezwa okungalungile kwe-eluent.

Isiphakamiso: Qiniseka ukuthi i-ddH2O Engenayo i-RNase yengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza, futhi ungayibeki eringini yekholomu yokuhlanza.

5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RW2.

Ukusikisela: Sicela ulandele imiyalelo, wengeze ivolumu efanele ye-ethanol ephelele ku-Buffer RW2 futhi uhlanganise kahle ngaphambi kokusebenzisa ikhithi.

6. Ivolumu yesampula engafanele.

Isiphakamiso: U-200µl wesampula ucutshungulwa kuwo wonke u-500µl we-Buffer DRL.Ukucutshungulwa kwesampula okweqile kuzoholela emvuzweni ephansi yokukhipha i-nucleic acid.

7. Ivolumu ye-elution engafanele noma ukungezwani okuphelele.

Isincomo: Ivolumu ecacile yekholomu yokuhlanza ingu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi unwebe isikhathi kuzinga lokushisa legumbi ngemva kokwengeza i-ddH2O eshisiwe ye-RNase-Free, njenge-5-10min.

8. I-Ethanol ihlala kukholamu ngemva kokugeza nge-Buffer RW2.

Isiphakamiso: Uma i-ethanol isala ngemva kokufakwa phakathi kwe-centrifugation ne-Buffer RW2 imizuzu emi-2, ikholomu ingabekwa ekamelweni lokushisa imizuzu emi-5 ngemva kwe-centrifugation ukuze ikhiphe ngokuphelele i-ethanol esele.

I-nucleic acid ehlanzekile iyancipha

Izinga le-nucleic acid ehlanzekile lihlobene nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, ukusebenza nezinye izici.Ukuhlaziywa kwezimbangela ezijwayelekile:

1. Amasampula aqoqiwe awagcinwanga ngesikhathi.

Ukusikisela: Uma isampuli ingasetshenziswa ngesikhathi ngemva kokuqoqwa, sicela uyigcine ku -80°C ezingeni lokushisa eliphansi ngokushesha.Ukuze uthole i-RNA, zama ukusebenzisa amasampuli asanda kuqoqwa.

2. Qoqa amasampula bese uwaqandisa futhi uncibilike ngokuphindaphindiwe.

Ukusikisela: Gwema ukuqanda nokuncibilika (hhayi ngaphezu kokukodwa) ngesikhathi sokuqoqwa nokugcinwa kwamasampula, ngaphandle kwalokho isivuno se-nucleic acid sizoncipha.

3. I-RNase yethulwa egumbini lokuhlinza noma amagilavu ​​alahlwayo, imaski, njll. awagqokwa.

Okutuswayo: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu ekamelweni lokusebenza elihlukile le-RNA, futhi itafula laselabhorethri kufanele lihlanzwe ngaphambi kokuhlolwa.

Gqoka amagilavu ​​alahlwayo namamaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase ngezinga elikhulu kakhulu.

4. I-reagent yonakaliswe i-RNase ngesikhathi sokusetshenziswa.

Isincomo: Faka esikhundleni se-Viral DNA/RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.

5. Amashubhu e-centrifuge namathiphu e-piette asetshenziselwa ukukhwabanisa kwe-RNA angcoliswe yi-RNase.

Ukusikisela: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu e-pipette, amapayipi, njll. asetshenziselwa ukukhipha i-RNA konke Akuna-RNAse.

I-nucleic acid ehlanjululwe ithinta ukuhlolwa okungezansi komfula

I-DNA ne-RNA ehlanjululwe yikholomu yokuhlanza, uma i-ion kasawoti kanye nokuqukethwe kwamaprotheni kuphezulu kakhulu, kuzothinta ukuhlolwa okuya phansi komfula, okufana nalokhu: Ukukhulisa i-PCR, ukuloba okuhlanekezelwe, njll.

1. I-DNA ekhishiwe kanye ne-RNA inama-ion kasawoti asele.

Isiphakamiso: Qinisekisa ukuthi ivolumu elungile ye-ethanol ephelele yengezwa ku-Buffer RW2, futhi ugeze ikholomu yokuhlanza kabili ngesivinini sokuhlanganisa esishiwo emiyalweni yokusebenza;Yenza i-centrifugation ukuze unciphise ukungcoliswa kwe-ion kasawoti.

2. I-DNA ekhishiwe kanye ne-RNA inezinsalela ze-ethanol.

Ukusikisela: Ngemva kokuqinisekisa ukugeza nge-Buffer RW2, yenza i-tube centrifugation engenalutho ngesivinini se-centrifugation emiyalweni yokusebenza;uma kusekhona izinsalela ze-ethanol, ungakwazi ukufaka i-centrifuge ishubhu elingenalutho bese ulibeka endaweni yokushisa yasekamelweni imizuzu engu-5 ukuze ususe insalela ye-ethanol ngezinga elikhulu kakhulu.

Amamanuwali wemiyalo:

I-Viral DNA&RNA Isolation Kit Instruction Manual