Ukuze kuhlangatshezwane nenjabulo elindelwe kakhulu yamakhasimende, manje sineqembu lethu eliqinile elizohlinzeka ngosizo lwethu olukhulu kunawo wonke oluhlanganisa ukumaketha, ukuthengisa, ukuhlela, ukukhiqiza, ukulawula izinga eliphezulu, ukupakisha, ukugcinwa kwempahla kanye nempahla yefekthri edayiswa ngokushisayo I-Magnetic Bead Nucleic Acid Extraction Kits Rna Isolation DNA Purification PCR Kit, "Shintsha Ikhithi Yokuhlola I-PCR engcono kakhulu!"isiqubulo sethu, esisho ukuthi “Imbulunga engcono iphambi kwethu, ngakho asiyithande!”Shintsha ube ngcono kakhulu!Senilungile?
Ukuze kuhlangatshezwane nenjabulo elindelwe ngamakhasimende, manje sineqembu lethu eliqinile elizohlinzeka ngosizo lwethu olukhulu kunawo wonke oluhlanganisa ukumaketha, ukuthengisa, ukuhlela, ukukhiqiza, ukulawula izinga eliphezulu, ukupakisha, ukugcinwa kwempahla kanye nokuhlelwa kwempahla.I-China Magnetic Bead kanye nekhithi yokuxilonga, Okuhlangenwe nakho kwethu kusenza sibaluleke emehlweni ethu amakhasimende.Ikhwalithi yethu izikhulumela ngokwazo izici njengokuthi ayihlangani, ayichithi noma ayiwohloki, ngakho amakhasimende ethu azohlala eqiniseka ngenkathi efaka i-oda.

Izincazelo zekhithi

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu yebhafa eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Imininingwane

*: I-Buffer L2 kanye ne-Buffer PW iqukethe usawoti omuncu ocikayo.Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside.

Ukuze kuhlangatshezwane nenjabulo elindelwe kakhulu yamakhasimende, manje sineqembu lethu eliqinile elizohlinzeka ngosizo lwethu olukhulu kunawo wonke oluhlanganisa ukumaketha, ukuthengisa, ukuhlela, ukukhiqiza, ukulawula izinga eliphezulu, ukupakisha, ukugcinwa kwempahla kanye nempahla yefekthri edayiswa ngokushisayo I-Magnetic Bead Nucleic Acid Extraction Kits Rna Isolation DNA Purification PCR Kit, "Shintsha Ikhithi Yokuhlola I-PCR engcono kakhulu!"isiqubulo sethu, esisho ukuthi “Imbulunga engcono iphambi kwethu, ngakho asiyithande!”Shintsha ube ngcono kakhulu!Senilungile?
Ifekthri yenziwe yadayiswa kakhuluI-China Magnetic Bead kanye nekhithi yokuxilonga, Okuhlangenwe nakho kwethu kusenza sibaluleke emehlweni ethu amakhasimende.Ikhwalithi yethu izikhulumela ngokwazo izici njengokuthi ayihlangani, ayichithi noma ayiwohloki, ngakho amakhasimende ethu azohlala eqiniseka ngenkathi efaka i-oda.