Ngokunamathela enkolelweni “Yokudala izinto ezisezingeni eliphezulu nokudala ubungane nabantu namuhla abavela emhlabeni wonke”, sivame ukubeka isithakazelo sabathengi kuqala ku-Supply ODM China CE FDA Compatible.Ukukhishwa kwe-Nucleic AcidUkuhlolwa kwe-Reagent Kit 96, Uma uzingela kanye Ikhwalithi ephezulu ngentengo enhle kakhulu kanye nokulethwa okufika ngesikhathi.Xhumana nathi.
Ukunamathela enkolelweni “yokudala izinto ezisezingeni eliphezulu nokudala ubungane nabantu namuhla abavela kuwo wonke umhlaba”, sivame ukubeka izithakazelo zabathengi kuqalaI-China Nucleic Acid Extraction Reagent, Ukukhishwa kwe-Nucleic Acid, Manje sinemisebenzi engaphezu kwe-100 embonini, futhi sineqembu labafana abangu-15 elizosebenzela amakhasimende ethu ngaphambi nangemuva kokuthengisa.Ikhwalithi enhle iyisici esibalulekile sokuthi inkampani ihluke kwezinye izimbangi.Ukubona ukuthi Uyakholwa, ufuna ulwazi olwengeziwe?Isivivinyo nje sezimpahla zakho!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Ukunamathela enkolelweni "Yokudala izinto ezisezingeni eliphezulu nokudala ubungane nabantu namuhla abavela kuwo wonke umhlaba", sivame ukubeka isithakazelo sabathengi endaweni yokuqala ye-Supply ODM China CE FDA Compatible Nucleic Acid Extraction Reagent Kit 96 Tests, Uma uzingela kanye Ikhwalithi ephezulu ngentengo enhle kakhulu kanye nokulethwa ngesikhathi.Xhumana nathi.
Thenga i-ODMI-China Nucleic Acid Extraction Reagent, I-Nucleic Acid Extraction, Manje sinemisebenzi engaphezu kwe-100 embonini, futhi sineqembu labafana abangu-15 elisebenzela amakhasimende ethu ngaphambi nangemuva kokuthengisa.Ikhwalithi enhle iyisici esibalulekile sokuthi inkampani ihluke kwezinye izimbangi.Ukubona ukuthi Uyakholwa, ufuna ulwazi olwengeziwe?Isivivinyo nje sezimpahla zakho!