Isixazululo SaseShayina Esingasebenzi Esisebenzayo Sokulondoloza Igciwane Nge-Rnase Inhibitors
Ngendlela ethembekile yekhwalithi enhle, irekhodi elimangalisayo kanye nesevisi ephelele yabathengi, uchungechunge lwezixazululo ezikhiqizwa ibhizinisi lethu zithunyelwa emazweni amaningi nasezifundeni ze-Chinese Professional Inactivated Efficient Virus Preservation Solution nge-Rnase Inhibitors, Futhi singasiza ekufuneni noma yimiphi imikhiqizo yezidingo zamakhasimende.Qiniseka ukuthi unikeza Isevisi engcono kakhulu, Ikhwalithi Engcono Kakhulu, Ukulethwa okusheshayo.
Ngendlela ethembekile yekhwalithi enhle, irekhodi elimangalisayo kanye nesevisi ephelele yabathengi, uchungechunge lwezixazululo ezikhiqizwa ibhizinisi lethu zithunyelwa emazweni amaningi nasezifundeniI-China Virus Preservation ene-Rnase Inhibitors kanye ne-Inactivated Virus Preservation Reagent, Kunoma ubani othanda noma yiziphi izimpahla zethu ngemva nje kokubuka uhlu lwemikhiqizo yethu, sicela uzizwe ukhululekile ngokuphelele ukuxhumana nathi ukuze uthole imibuzo.Uyakwazi ukusithumelela ama-imeyili futhi usithinte ukuze kuboniswane futhi sizokuphendula ngokushesha ngangokunokwenzeka.Uma kulula, ungase uthole ikheli lethu kusizindalwazi sethu futhi uze ebhizinisini lethu ukuze uthole ulwazi olwengeziwe lwezisombululo zethu ngokwakho.Besihlale sikulungele ukwakha ubudlelwano bokubambisana obandisiwe nobuzinzile nanoma imaphi amakhasimende angaba khona emikhakheni ehlobene.
Imininingwane
5ml, 10ml, 40ml
I-PCR Hero (Nge-Dye) Ikhithi yokukhipha ihlanganisa isizukulwane esisha esiyingqayizivele sika-Foregene se-Taq—Foregene Taq Hero, ama-dNTP athuthukisiwe, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer nesiqinisi.Uhlelo lwe-2× PCR Hero TM Mix (udayi) lunokubekezelela okuphezulu kwe-PCR inhibitors kunohlelo olujwayelekile lwe-PCR Mix, futhi lungabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
Izingxenye zekhithi
0.1U Taq Iqhawe/μl |
400μM dNTPs |
20mM Tris-HCl(pH8.8) |
100mM KCl |
5mM MgCl2 |
Amanye ama-stabilizer kanye nezithuthukisi |
I-DNA Loading Buffer |
Izici&izinzuzo
■ Ukwethembeka okuphezulu: izikhathi eziyisi-6 kune-enzyme ye-Taq evamile;
■ Isivinini sokukhulisa esisheshayo : ijubane lokukhulisa ngu-5-10sec/kb, eliphindwe izikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;
■ Ukuguquguquka okwengeziwe kwesifanekiso: Ingakwazi ukukhulisa izifanekiso ze-DNA eziyinkimbinkimbi ngokuphezuluOkuqukethwe kwe-GC;
■ Ukusebenza okuphakeme kokukhulisa amandla: imijikelezo yokukhulisa ephansi kune-enzyme ye-Taq evamile;
∎ Ukubekezelelana kwemvelo kunamandla: ibekwe ku-37°C isonto lonke, igcina umsebenzi ongaphezu kwama-90%;
■ Inomsebenzi ongu-5′ →3′ we-DNA polymerase kanye no-5′→3′ umsebenzi we-exonuclease, ngaphandle komsebenzi we-3′ →5′ we-exonuclease.
■ Ikhithi ixutshwe ngaphambili ne-Electrophoresis Loading Buffer, ngemva kokuba i-PCR isiqediwe, isampula ingalayishwa ngokuqondile i-electrophoresis;ukusebenza kulula futhi kulula.
Isicelo sekhithi
I-PCR yokukhulisa izingcezu ze-DNA, ukulebula i-DNA, ukulandelana kwe-DNA,I-PCR kanye nomsila we-A
Isitoreji kanye Nempilo yeshelufu
Ingagcinwa isikhathi eside ku -20°C;ukuze isetshenziswe njalo, ingagcinwa ku-4°C isikhathi esifushane.Ngendlela ethembekile yekhwalithi enhle, irekhodi elimangalisayo kanye nesevisi ephelele yabathengi, uchungechunge lwezixazululo ezikhiqizwa ibhizinisi lethu zithunyelwa emazweni amaningi nasezifundeni ze-Chinese Professional Inactivated Efficient Virus Preservation Solution nge-Rnase Inhibitors, Futhi singasiza ekufuneni noma yimiphi imikhiqizo yezidingo zamakhasimende.Qiniseka ukuthi unikeza Isevisi engcono kakhulu, Ikhwalithi Engcono Kakhulu, Ukulethwa okusheshayo.
Uchwepheshe waseShayinaI-China Virus Preservation ene-Rnase Inhibitors kanye ne-Inactivated Virus Preservation Reagent, Kunoma ubani othanda noma yiziphi izimpahla zethu ngemva nje kokubuka uhlu lwemikhiqizo yethu, sicela uzizwe ukhululekile ngokuphelele ukuxhumana nathi ukuze uthole imibuzo.Uyakwazi ukusithumelela ama-imeyili futhi usithinte ukuze kuboniswane futhi sizokuphendula ngokushesha ngangokunokwenzeka.Uma kulula, ungase uthole ikheli lethu kusizindalwazi sethu futhi uze ebhizinisini lethu ukuze uthole ulwazi olwengeziwe lwezisombululo zethu ngokwakho.Besihlale sikulungele ukwakha ubudlelwano bokubambisana obandisiwe nobuzinzile nanoma imaphi amakhasimende angaba khona emikhakheni ehlobene.