Cabanga “ngeKhasimende kuqala, Kuhle kakhulu kuqala”, sisebenzisana eduze namakhasimende ethu futhi siwanikeze ngezinsizakalo ezisebenza kahle nezingochwepheshe zokudayiswa kwefektri.I-China Nucleic Acid Extraction ReagentI-Rna Extraction Test Kits, Ifemu yethu izinikele ekunikezeni amakhasimende izinto zekhwalithi ephezulu neziphephile ngezindleko ezincintisanayo, ezuza wonke amakhasimende aneliseke ngamasevisi ethu.
Cabanga “ngeKhasimende kuqala, Kuhle kakhulu kuqala”, sisebenzisana eduze namakhasimende ethu futhi siwanikeze izinsiza ezisebenza kahle nezingochwephesheI-China Nucleic Acid Extraction Reagent, Izinto zethu zibonwa kabanzi futhi zithenjwa ngabasebenzisi futhi zingahlangabezana ngokuqhubekayo nezidingo zezomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze athole ubudlelwano bebhizinisi besikhathi esizayo futhi azuze impumelelo efanayo!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela).Bear “Ikhasimende kuqala, Kuhle kakhulu kuqala” engqondweni, sisebenzisana eduze namakhasimende ethu futhi siwanikeze ngezinsizakalo ezisebenza kahle nezingochwepheshe zeFactory wholesale yaseChina Nucleic Acid Extraction Reagent (Serum/Plasma, I-Plasma ne-Puncture evikelekile enikeza amakhasimende ethu Isixazululo se-Kimsho kanye ne-Puncture evikelekile) izinto zekhwalithi ephezulu ngezindleko ezincintisanayo, ezizuza wonke amakhasimende aneliseke ngamasevisi ethu.
I-Factory wholesale yase-China Nucleic Acid Extraction Reagent, Indlela Ye-Magnetic Bead, Izinto zethu zibonwa kabanzi futhi zithenjwa ngabasebenzisi futhi zingahlangabezana ngokuqhubekayo nezidingo zomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze athole ubudlelwano bebhizinisi besikhathi esizayo futhi azuze impumelelo efanayo!