Umsebenzi wethu kungaba ukusiza amakhasimende ethu kanye namakhasimende ethu ngemikhiqizo yedijithali ehamba phambili kakhulu futhi enolaka Yabahlinzeki Bempahla Abaphezulu base-China Hot Sale Rna Extraction Kit Lab Research Reagents, Manje sesihlangabezane nezindawo zokukhiqiza ezinezisebenzi eziningi ezingaphezu kwe-100.Ngakho siyakwazi ukuqinisekisa isikhathi esifushane sokuhola kanye nokuqinisekiswa kwekhwalithi enhle.
Umsebenzi wethu kungaba ukusebenzela amakhasimende ethu kanye namakhasimende ethu ngemikhiqizo yedijithali ehamba phambili kakhulu futhi enolakaI-China Rna Isolation Reagents, Singanikeza amakhasimende ethu izinzuzo eziphelele ngekhwalithi yomkhiqizo kanye nokulawulwa kwezindleko, futhi manje sinohlu olugcwele lwesikhunta kusukela kumafekthri afinyelela kwekhulu.Njengokubuyekezwa komkhiqizo ngokushesha, siphumelela ekwakheni imikhiqizo eminingi yekhwalithi ephezulu yamakhasimende ethu futhi sithole idumela eliphezulu.

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ikhomishana yethu kuyoba ukunikeza amakhasimende ethu kanye namakhasimende ethu ngemikhiqizo yedijithali ehamba phambili kakhulu futhi enolaka ephathekayo Yabaphakeli Abaphezulu I-China Hot Sale Rna Nucleic Acid Extraction Kit Lab Reagents Yokucwaninga, manje sinezisebenzi eziningi ezinolwazi oluningi kunezindawo zokukhiqiza eziyi-1.Ngakho siyakwazi ukuqinisekisa isikhathi esifushane sokuhola kanye nokuqinisekiswa kwekhwalithi enhle.
Abahlinzeki Abaphezulu baseChina I-Rna Nucleic Acid Isolation Reagents, I-Nucleic Acid Detection Kits, Singanikeza amakhasimende ethu izinzuzo eziphelele ngekhwalithi yomkhiqizo nokulawulwa kwezindleko, futhi manje sesinohlu olugcwele lwesikhunta kusukela ezimbonini eziyikhulu.Njengokubuyekezwa komkhiqizo ngokushesha, siphumelela ekwakheni imikhiqizo eminingi yekhwalithi ephezulu yamakhasimende ethu futhi sithole idumela eliphezulu.