Ibhizinisi lethu selokhu lasungulwa, livame ukubheka isisombululo esihle njengempilo yebhizinisi, liqhubeka liqinisa ubuchwepheshe bokuphuma, lithuthukisa izinga eliphezulu lomkhiqizo futhi liqinise njalo inhlangano yonke yokuphatha okusezingeni eliphezulu, ngokuhambisana ngokuqinile kusetshenziswa indinganiso kazwelonke ye-ISO 9001:2000 ngentengo ecashuniwe ye-China CE Igunyazwe i-Nucleic Acid 64 Well Plate DNA Rna Extraction Isolation Kit yemikhiqizo yethu enentshisekelo yokuthola i-PC Diragnostic Isolation Kit, Siyakwamukela ku-PC yethu I-Diagnostic Kit sizokunikeza i-surprice ye-Qulity ne-Price.
Ibhizinisi lethu selokhu lasungulwa, livame ukubheka isisombululo esihle njengempilo yebhizinisi, liqhubeka liqinisa ubuchwepheshe obuphumayo, lithuthukisa izinga eliphezulu lomkhiqizo futhi liqinise njalo inhlangano yonke yokuphathwa kwekhwalithi ephezulu, ngokuhambisana ngokuqinile kusetshenziswa indinganiso kazwelonke ye-ISO 9001:2000I-China Viral Nucleic Acid Isolation, I-Real Time PCR Diagnostic, Sithatha izilinganiso nganoma yiziphi izindleko ukuze sizuze okokusebenza kanye nezindlela ezisesikhathini samanje.Ukupakishwa komkhiqizo okhethiwe kuyisici sethu esengeziwe esihlukanisayo.Imikhiqizo yokuqinisekisa iminyaka yesevisi engenazinkinga ihehe amakhasimende amaningi.Izixazululo zitholakala ngemiklamo ethuthukisiwe kanye ne-assortment ecebile, zidalwe ngokwesayensi ngezinto ezingavuthiwe kuphela.Itholakala kalula ngemiklamo ehlukahlukene kanye nokucaciswa kokukhetha kwakho.Izinhlobo zakamuva zingcono kakhulu kunezo ezandulelayo futhi zithandwa kakhulu ngamathemba amaningi.

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ibhizinisi lethu kusukela lasungulwa, livame ukubheka isisombululo esihle njengempilo yebhizinisi, lihlale liqinisa ubuchwepheshe obuphumayo, lithuthukisa izinga eliphezulu lomkhiqizo futhi lihlale liqinisa ukuphathwa kwenhlangano kwekhwalithi ephezulu, ngokuhambisana ngokuqinile kusetshenziswa indinganiso kazwelonke ye-ISO 90001 ye-Account Quote 4 ye-Account Quoc 4 yaseChina I-Plate DNA Rna Extraction Isolation Kit ye-PCR Virus Rapid Diagnostic, Siyakwamukela ukuze usibambe kunoma ubani onentshisekelo emikhiqizweni yethu, sizokunikeza i-surprice ye-Qulity ne-Price.
Intengo ecashuniwe yeI-China Viral Nucleic Acid Isolation, I-Real Time PCR Diagnostic, Sithatha izilinganiso nganoma yiziphi izindleko ukuze sizuze okokusebenza kanye nezindlela ezisesikhathini samanje.Ukupakishwa komkhiqizo okhethiwe kuyisici sethu esengeziwe esihlukanisayo.Imikhiqizo yokuqinisekisa iminyaka yesevisi engenazinkinga ihehe amakhasimende amaningi.Izixazululo zitholakala ngemiklamo ethuthukisiwe kanye ne-assortment ecebile, zidalwe ngokwesayensi ngezinto ezingavuthiwe kuphela.Itholakala kalula ngemiklamo ehlukahlukene kanye nokucaciswa kokukhetha kwakho.Izinhlobo zakamuva zingcono kakhulu kunezo ezandulelayo futhi zithandwa kakhulu ngamathemba amaningi.