Ukuthuthukiswa kwethu kuncike emishinini ephakeme, amathalente amahle kakhulu kanye namandla obuchwepheshe aqiniswa ngokuqhubekayokhipha i-RNA ephelele, Ukuze ufunde okwengeziwe ngalokho esingakwenzela khona mathupha, khuluma nathi noma nini.Sibheke ngabomvu ukuthuthukisa izinhlangano zenhlangano ezinhle nezesikhathi eside kanye nawe.
Ukuthuthukiswa kwethu kuncike emishinini ephakeme, amathalente amahle kakhulu kanye namandla obuchwepheshe aqiniswa ngokuqhubekayokhipha i-RNA ephelele, Isebenzisa uhlelo oluhamba phambili emhlabeni lokusebenza okuthembekile, izinga lokwehluleka eliphansi, ilungele ukukhetha kwamakhasimende ase-Argentina.Inkampani yethu itholakala emadolobheni kazwelonke aphucuzekile, ithrafikhi ilula kakhulu, izimo zendawo nezomnotho zihlukile.Siphishekela ukuthambekela kwabantu, ukukhiqiza okucophelelayo, ukuxoxisana, ukwakha ifilosofi yebhizinisi ekhaliphile.Ukuphathwa kwekhwalithi eqinile, isevisi ephelele, intengo enengqondo e-Argentina kuyisimo sethu esisekelweni sokuncintisana.Uma kunesidingo, wamukelekile ukuthi usithinte ngewebhusayithi yethu noma ngokubonisana ngocingo, sizokujabulela ukukusebenzela.

Imininingwane

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Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer CRL1 ingagcinwa ngo-4 ℃ ngenyanga engu-1 ngemuva kokungeza i-2-hydroxy-1-ethaneniol (inketho yezobuchwepheshe ixhomeke efemini ephakeme yokuphulukisa isikhumba, ukuze ufunde kabanzi ngalokho esingakwenzela kona uqobo, khuluma nathi nganoma yisiphi isikhathi.Sibheke ngabomvu ukuthuthukisa izinhlangano zenhlangano ezinhle nezesikhathi eside kanye nawe.
I-Professional Factory for China Amino Acid, Pharmaceutical, Isebenzisa uhlelo oluhamba phambili emhlabeni lokusebenza okuthembekile, izinga eliphansi lokwehluleka, ifanele ukukhetha kwamakhasimende ase-Argentina.Inkampani yethu itholakala emadolobheni kazwelonke aphucuzekile, ithrafikhi ilula kakhulu, izimo zendawo nezomnotho zihlukile.Siphishekela ukuthambekela kwabantu, ukukhiqiza okucophelelayo, ukuxoxisana, ukwakha ifilosofi yebhizinisi ekhaliphile.Ukuphathwa kwekhwalithi eqinile, isevisi ephelele, intengo enengqondo e-Argentina kuyisimo sethu esisekelweni sokuncintisana.Uma kunesidingo, wamukelekile ukuthi usithinte ngewebhusayithi yethu noma ngokubonisana ngocingo, sizokujabulela ukukusebenzela.