Sinamakhasimende amaningi abasebenzi abahle kakhulu ekukhangiseni, i-QC, nokusebenza ngezinhlobo zobunzima obuyinkathazo ngaphakathi kwendlela yokukhiqiza ye-Factory wholesale yase-China Lab Nucleic Acid Extraction DNA Rna Purification Isolation Kit ene-CE Mark., Silwela kanzima ukuzuza impumelelo eqhubekayo ngokwekhwalithi enhle, ukwethembeka, ubuqotho, nokuqonda okuphelele kokuguquguquka komkhakha.
Sinamakhasimende amaningi abasebenzi abahle kakhulu ekukhangiseni, i-QC, nokusebenza ngezinhlobo zobunzima obunzima ngaphakathi kwendlela yokukhiqizaI-China Nucleic Acid Isolation Kit, I-DNA Rna Isolation Kit, Idizayini, ukucutshungulwa, ukuthenga, ukuhlola, ukugcinwa, inqubo yokuhlanganisa konke kusenqubweni yemibhalo yesayensi nephumelelayo, ukwandisa izinga lokusetshenziswa nokuthembeka komkhiqizo wethu ngokujulile, okusenza sibe abahlinzeki abaphakeme bezigaba ezine zemikhiqizo emikhulu yokusakazwa kwamagobolondo ekhaya futhi sathola ukwethenjwa kwekhasimende kahle.

Imininingwane

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Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu ye-buffer eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15–25 ℃) kanye no-2–8 ℃ isikhathi eside. Sinamakhasimende amaningi amahle kakhulu asebenza kahle ekukhangiseni, i-QC, nokusebenza ngezinhlobo zobunzima obuyinkathazo ngaphakathi kwendlela yokukhiqiza ye-Factory wholesale China Lab 64 Well Plate Testlation Magnetic Bead Nucleic DNA, I-Kitnaid Exporation impumelelo eqhubekayo ngokuya ngekhwalithi enhle, ukwethembeka, ubuqotho, nokuqonda okuphelele kokuguquguquka komkhakha.
I-Factory wholesaleI-China Nucleic Acid Isolation Kit, I-DNA Rna Isolation Kit, Idizayini, ukucutshungulwa, ukuthenga, ukuhlola, ukugcinwa, inqubo yokuhlanganisa konke kusenqubweni yemibhalo yesayensi nephumelelayo, ukwandisa izinga lokusetshenziswa nokuthembeka komkhiqizo wethu ngokujulile, okusenza sibe abahlinzeki abaphakeme bezigaba ezine zemikhiqizo emikhulu yokusakazwa kwamagobolondo ekhaya futhi sathola ukwethenjwa kwekhasimende kahle.