Ukwaneliseka kweklayenti yikona esigxile kukho.Siphakamisa izinga elingaguquki lobungcweti, ikhwalithi ephezulu, ukwethembeka kanye nensizakalo ye-Hipure PCR Pure Mini Kit Recover DNA Fragments Phakathi kuka-60bp-20kbp, Siyakwamukela ukuphequlula kwakho nanoma yimiphi imibuzo yakho, ngethemba lobuqotho sizokwazi ukuba nethuba lokubambisana nawe futhi sizokwakha umshado ngempumelelo.
Ukwaneliseka kweklayenti yikona esigxile kukho.Siphakamisa izinga elingaguquki lobuchwepheshe, ikhwalithi ephezulu, ukwethembeka kanye nesevisi yeI-China Rna/DNA Extraction Purification kanye ne-Nucleic Acid Isolation, Sisungule ubudlelwano bebhizinisi besikhathi eside, obuzinzile futhi obuhle nabakhiqizi nabathengisi abaningi emhlabeni jikelele.Njengamanje, besibheke phambili ekubambisaneni okukhulu nakakhulu namakhasimende aphesheya okusekelwe ezinzuzweni ezifanayo.Qiniseka ukuthi uzizwa ukhululekile ukusithinta ukuze uthole imininingwane eyengeziwe.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo

∎ Isidingo sesampula sincane, njengoba izicubu zezilwane ezingama-5mg zingahlolwa.

■ Akukho ukwelashwa okukhethekile okufana nokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: izicubu ezihlukahlukene zezilwane.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer AL ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2 × PCR Easy^TMImiksi ingagcinwa izinyanga eziyi-12 ku -20℃;uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).

Ukwaneliseka kweklayenti yikona esigxile kukho.Siphakamisa izinga elingaguquki lobungcweti, ikhwalithi ephezulu, ukwethembeka kanye nensizakalo ye-Hipure PCR Pure Mini Kit Recover DNA Fragments Phakathi kuka-60bp-20kbp, Siyakwamukela ukuphequlula kwakho nanoma yimiphi imibuzo yakho, ngethemba lobuqotho sizokwazi ukuba nethuba lokubambisana nawe futhi sizokwakha umshado ngempumelelo.
Iklanywe kahleI-China Rna/DNA Extraction Purification kanye ne-Nucleic Acid Isolation, Sisungule ubudlelwano bebhizinisi besikhathi eside, obuzinzile futhi obuhle nabakhiqizi nabathengisi abaningi emhlabeni jikelele.Njengamanje, besibheke phambili ekubambisaneni okukhulu nakakhulu namakhasimende aphesheya okusekelwe ezinzuzweni ezifanayo.Qiniseka ukuthi uzizwa ukhululekile ukusithinta ukuze uthole imininingwane eyengeziwe.