Inhloso yethu kuzoba ukugcwalisa abathengi bethu ngokunikeza usizo lwegolide, intengo enhle kanye nekhwalithi ephezulu Yekhwalithi Ephezulu ye-China 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit ene-Magnetic Bead Method Yokutholwa kwe-PCR, Manje sineqembu elinekhono lohwebo lwamazwe ngamazwe.Sizoxazulula inkinga ohlangana nayo.Singethula izimpahla ozifunayo.Qiniseka ukuthi uzizwa ukhululekile ukuxhumana nathi.
Inhloso yethu kuzoba ukugcwalisa abathengi bethu ngokunikeza usizo lwegolide, intengo enhle kanye nekhwalithi ephezulu yeI-China DNA Rna Isolation, Ukuhlukaniswa kwe-Viral Nucleic Acid, Isevisi esheshayo nenolwazi emva kokuthengisa ehlinzekwa yiqembu lethu labaxhumanisi ibathokozisile abathengi bethu.Ulwazi oluningiliziwe namapharamitha asuka kokuthengiswayo cishe azothunyelwa kuwe nganoma ikuphi ukuvuma okuphelele.Amasampula wamahhala angase alethwe futhi inkampani ihlole enkampanini yethu.n I-Morocco ngezingxoxo yamukelekile njalo.Ngethemba lokuthola imibuzo ukuthayipha bese wakhe ubambiswano lwesikhathi eside.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

Inhloso yethu kuzoba ukugcwalisa abathengi bethu ngokunikeza usizo lwegolide, intengo enhle kanye nekhwalithi ephezulu Yekhwalithi Ephezulu ye-China 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit ene-Magnetic Bead Method Yokutholwa kwe-PCR, Manje sineqembu elinekhono lohwebo lwamazwe ngamazwe.Sizoxazulula inkinga ohlangana nayo.Singethula izimpahla ozifunayo.Qiniseka ukuthi uzizwa ukhululekile ukuxhumana nathi.
Ikhwalithi ephezulu yeI-China DNA Rna Isolation, Ukuhlukaniswa kwe-Viral Nucleic Acid, Isevisi esheshayo nenolwazi emva kokuthengisa ehlinzekwa yiqembu lethu labaxhumanisi ibathokozisile abathengi bethu.Ulwazi oluningiliziwe namapharamitha asuka kokuthengiswayo cishe azothunyelwa kuwe nganoma ikuphi ukuvuma okuphelele.Amasampula wamahhala angase alethwe futhi inkampani ihlole enkampanini yethu.n I-Morocco ngezingxoxo yamukelekile njalo.Ngethemba lokuthola imibuzo ukuthayipha bese wakhe ubambiswano lwesikhathi eside.