Abahlinzeki Abaphezulu baseChina I-PCR ye-Nucleic Acid Extraction System ezenzakalelayo, Izisetshenziswa Zokudonsa I-Acid Nucleic
Incazelo yekhithi:
I-RNase-Free
Isebenza ekamelweni lokushisa (15-25 ℃)
Ngekholomu Yokuhlanza I-DNA
Inani eliphakeme kakhulu lama-RNA
Ukushesha:Qeda ukukhipha emizuzwini eyi-11
Ukuphepha:Ayikho i-Organic chemical
Sithola injabulo ngokuduma okuhle ngendlela emangalisayo phakathi kwamakhasimende ethu ngokuthengiswa kwethu okuhle kwekhwalithi ephezulu, isilinganiso esinolaka kanye nokwesekwa okuphumelelayo kwe-Top Suppliers China Automated PCR.Ukukhishwa kwe-Nucleic AcidIsistimu, Izisetshenziswa Ze-Acid Nucleic Extraction, Ukuphepha ngenxa yokuqanjwa kabusha kuyisithembiso sethu komunye nomunye.
Sithola injabulo ngokuduma okuhle ngendlela emangalisayo phakathi kwamakhasimende ethu ngokuthengiswa kwethu okuhle kwekhwalithi ephezulu, izinga elinamandla kanye nokusekelwa okusebenzayoI-China Nucleic Acid Extraction Machine, Ukukhishwa kwe-Nucleic Acid, Sihlinzeka ngenkonzo yobungcweti, impendulo esheshayo, ukulethwa okufika ngesikhathi, ikhwalithi enhle kakhulu kanye nentengo engcono kakhulu kumakhasimende ethu.Ukwaneliseka kanye nekhredithi enhle kuwo wonke amakhasimende kuyinto ehamba phambili yethu.Sigxila kuyo yonke imininingwane yokucutshungulwa kwe-oda kumakhasimende aze athole izixazululo eziphephile nezizwakalayo ezinesevisi enhle yezokuthutha kanye nezindleko zokonga.Kuye ngalokhu, imikhiqizo yethu nezisombululo zithengiswa kahle kakhulu emazweni ase-Afrika, eMid-East naseNingizimu-mpumalanga Asia.Ngokuhambisana nefilosofi yebhizinisi 'yekhasimende kuqala, qhubekela phambili', samukela ngobuqotho amakhasimende asekhaya nakwamanye amazwe ukuba abambisane nathi.
Incazelo
50 Preps, 200 Preps
Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.
Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.
Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.
Izingxenye zekhithi
| Ukwakheka kwekhithi | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| I-Buffer cRL1* | 25 ml | 100 ml |
| Gcina i-cRL2 | 15 ml | 60 ml |
| Isilondolozi RW1* | 25 ml | 100 ml |
| Isilondolozi RW2 | 24 ml | 96 ml |
| RNase-Free ddH2O | 10 ml | 40 ml |
| Ikholomu Ye-RNA Kuphela | 50 | 200 |
| Ikholomu Yokuhlanza I-DNA | 50 | 200 |
| Isiyalezo | 1 | 1 |
*Sicela ugqoke amagilavu futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.
Izici&izinzuzo
■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.
Isicelo sekhithi
Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.
Ukugeleza komsebenzi
Umdwebo
Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.
Isitoreji nempilo yeshelufu
Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).
I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Sithola injabulo ngokuduma ngendlela emangalisayo phakathi kwamakhasimende ethu ngokuthengisa kwethu okuphambili kwekhwalithi ephezulu, izinga elinamandla kanye nokwesekwa okuphumelelayo kwe-Top Suppliers China Automated PCR.Ukukhishwa kwe-Nucleic AcidIsistimu, Izisetshenziswa Ze-Acid Nucleic Extraction, Ukuphepha ngenxa yokuqanjwa kabusha kuyisithembiso sethu komunye nomunye.
Abahlinzeki AbaphezuluI-China Nucleic Acid Extraction Machine, Isizinda Se-Nucleic Acid, Sihlinzeka ngensizakalo yobungcweti, impendulo esheshayo, ukulethwa okufika ngesikhathi, ikhwalithi enhle kakhulu kanye nentengo engcono kakhulu kumakhasimende ethu.Ukwaneliseka kanye nekhredithi enhle kuwo wonke amakhasimende kuyinto ehamba phambili yethu.Sigxila kuyo yonke imininingwane yokucutshungulwa kwe-oda kumakhasimende aze athole izixazululo eziphephile nezizwakalayo ezinesevisi enhle yezokuthutha kanye nezindleko zokonga.Kuye ngalokhu, imikhiqizo yethu nezisombululo zithengiswa kahle kakhulu emazweni ase-Afrika, eMid-East naseNingizimu-mpumalanga Asia.Ngokuhambisana nefilosofi yebhizinisi 'yekhasimende kuqala, qhubekela phambili', samukela ngobuqotho amakhasimende asekhaya nakwamanye amazwe ukuba abambisane nathi.
