Njengendlela yokukwethula kalula futhi sandise ibhizinisi lethu, sinabahloli ku-QC Staff futhi sikuqinisekisa inkampani yethu engcono kakhulu kanye nomkhiqizo wesampula Yamahhala Yefektri.I-China Viral Nucleic Acid (DNA/RNA) Ikhithi encane, Virus Rna Extraction Magnetic Beads V4002, 200 Preps, Sibheke phambili ekusunguleni izinhlangano zebhizinisi zesikhathi eside kanye nawe.Ukuphawula kwakho nezincomo zaziswa kakhulu.
Njengendlela yokukwethula kalula futhi sandise ibhizinisi lethu, sinabahloli ku-QC Staff futhi sikuqinisekisa inkampani yethu engcono kakhulu kanye nomkhiqizo(DNA/RNA) Ikhithi encane, I-China Viral Nucleic Acid, Sinikeza isevisi efanelekayo, impendulo esheshayo, ukulethwa ngesikhathi, ikhwalithi enhle kakhulu kanye nentengo engcono kakhulu kumakhasimende ethu.Ukwaneliseka kanye nekhredithi enhle kuwo wonke amakhasimende kuyinto ehamba phambili yethu.Sigxila kuyo yonke imininingwane yokucutshungulwa kwe-oda kumakhasimende aze athole imikhiqizo ephephile nezwakalayo enesevisi enhle yezokuthutha kanye nezindleko zokonga.Kuye ngalokhu, imikhiqizo yethu ithengiswa kahle kakhulu emazweni ase-Afrika, Mid-East naseNingizimu-mpumalanga Asia.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela).Njengendlela yokukwethula kalula futhi sandise ibhizinisi lethu, sinabahloli ku-QC Staff futhi sikuqinisekisa inkampani yethu engcono kakhulu kanye nomkhiqizo wesampula ye-Factory Free yase-China Viral Nucleic Acid.(DNA/RNA) Ikhithi encane, Virus Rna Extraction Magnetic Beads V4002, 200 Preps, Sibheke phambili ekusunguleni izinhlangano zebhizinisi zesikhathi eside kanye nawe.Ukuphawula kwakho nezincomo zaziswa kakhulu.
Isampula yamahhala yase-China Viral Nucleic Acid, (DNA/RNA) Miniprep Kit, Sinikeza isevisi efanelekayo, impendulo esheshayo, ukulethwa okufika ngesikhathi, ikhwalithi enhle kakhulu kanye nentengo engcono kakhulu kumakhasimende ethu.Ukwaneliseka kanye nekhredithi enhle kuwo wonke amakhasimende kuyinto ehamba phambili yethu.Sigxila kuyo yonke imininingwane yokucutshungulwa kwe-oda kumakhasimende aze athole imikhiqizo ephephile nezwakalayo enesevisi enhle yezokuthutha kanye nezindleko zokonga.Kuye ngalokhu, imikhiqizo yethu ithengiswa kahle kakhulu emazweni ase-Afrika, Mid-East naseNingizimu-mpumalanga Asia.