Sizibophezele ekunikezeni isevisi yokuthenga elula, eyonga isikhathi kanye neyonga imali eyodwa yokuthenga i-Top Quality Techstar Nucleic Acid Extraction System Iyathengiswa Umkhiqizi Wochungechunge Lwe-Nucleic Acid Ovela e-China, Sibheke phambili ekutholeni imibuzo yakho ngokushesha futhi sithemba ukuba nethuba lokwenza umsebenzi wenziwe nawe esikhathini esizayo.Siyakwamukela ukuze uthole ukubonakala enhlanganweni yethu.
Sizibophezele ekunikezeni isevisi yokuthenga yabathengi elula, eyonga isikhathi kanye neyonga imaliUhlelo lwe-China Nucleic Acid Extraction System, I-Nucleic Acid Extraction Instrument, Ngosekelo olungcono kakhulu lwezobuchwepheshe, senze iwebhusayithi yethu ifanelekela ulwazi lomsebenzisi olungcono kakhulu futhi sihlale sikhumbula kalula kwakho ukuthenga.siqinisekisa ukuthi okungcono kakhulu kukufinyelela emnyango wakho, ngesikhathi esifushane ngangokunokwenzeka nangosizo lozakwethu abaphumelelayo bokuhlela okuyi-DHL ne-UPS.Sithembisa ikhwalithi, siphila ngesiqubulo sokuthembisa kuphela lokho esingakuletha.

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Sizibophezele ekunikezeni isevisi yokuthenga elula, eyonga isikhathi kanye neyonga imali eyodwa yokuthenga i-Top Quality Techstar Nucleic Acid Extraction System Iyathengiswa Umkhiqizi Wochungechunge Lwe-Nucleic Acid Ovela e-China, Sibheke phambili ekutholeni imibuzo yakho ngokushesha futhi sithemba ukuba nethuba lokwenza umsebenzi wenziwe nawe esikhathini esizayo.Siyakwamukela ukuze uthole ukubonakala enhlanganweni yethu.
Ikhwalithi ephezuluUhlelo lwe-China Nucleic Acid Extraction System, I-Nucleic Acid Extraction Instrument, Ngosekelo olungcono kakhulu lwezobuchwepheshe, senze iwebhusayithi yethu ifanelekela ulwazi lomsebenzisi olungcono kakhulu futhi sihlale sikhumbula kalula kwakho ukuthenga.siqinisekisa ukuthi okungcono kakhulu kukufinyelela emnyango wakho, ngesikhathi esifushane ngangokunokwenzeka nangosizo lozakwethu abaphumelelayo bokuhlela okuyi-DHL ne-UPS.Sithembisa ikhwalithi, siphila ngesiqubulo sokuthembisa kuphela lokho esingakuletha.