Sibe ngumkhiqizi onolwazi.Ukuzuza iningi lezitifiketi zakho ezibalulekile zemakethe yayo ye-Super Purchasing for China Real-Time PCR, Uma kufanele ube nentshisekelo cishe kunoma yiziphi izimpahla, khumbula ukuzizwa ukhululekile ngokuphelele ukuxhumana nathi ukuze uthole amanye amaqiniso noma uqiniseke ukuthi usithumelela i-imeyili ngendlela efanele, sizokuphendula emahoreni angama-24 kanye nekhotheshini enhle kakhulu uzonikezwa.
Sibe ngumkhiqizi onolwazi.Ukuzuza iningi lezitifiketi zakho ezibalulekile zemakethe yayoI-PCR yaseChina, Isikhathi Sangempela Pcr, Ngomoya wokungenisa izihambi” wekhono eliphezulu, ukunethezeka, ukusebenziseka nokusungula izinto ezintsha”, futhi ngokuhambisana nesiqondiso esinjalo “sekhwalithi enhle kodwa amanani angcono, ” kanye “nesikweletu somhlaba wonke”, silwela ukubambisana nezinkampani zezingxenye zezimoto emhlabeni wonke ukuze senze ubambiswano oluwinayo.

Izincazelo

Le khithi isebenzisa isistimu ye-lysis buffer eyingqayizivele engakhulula ngokushesha i-RNA kumasampula eseli akhulisiwe ukuze kusabela i-RT-qPCR, ngaleyo ndlela iqede inqubo yokuhlanza i-RNA edla isikhathi nekhandlayo.Isifanekiso se-RNA singatholakala ngemizuzu engu-7 nje.I-5×Direct RT Mix kanye ne-2×Direct qPCR Mix-SYBR ama-reagents anikezwe ikhithi angathola ngokushesha nangempumelelo imiphumela ye-PCR yobuningi besikhathi sangempela.

I-5×Direct RT Mix kanye ne-2×Direct qPCR Mix-SYBR inokubekezelela okuqinile kwe-inhibitor, futhi i-lysate yamasampuli ingasetshenziswa njengesifanekiso se-RT-qPCR ngokuqondile.Le khithi iqukethe i-RNA high-affinity Foregene reverse transcriptase, kanye ne-Hot D-Taq DNA polymerase, dNTPs, MgCl₂, isigcinalwazi sokusabela, i-PCR optimizer nesiqinisi.

Imininingwane

200×20μl Rxns, 1000×20μl Rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Kulula futhi kusebenza ngempumelelo : Ngobuchwepheshe be-Cell Direct RT, amasampula e-RNA angatholwa ngemizuzu engu-7 nje.

■ Isidingo sesampula sincane, siphansi njengoba amaseli ayi-10 angahlolwa.

■ Ukuphuma okuphezulu: ikwazi ukubona ngokushesha i-RNA kumaseli akhiwe kumapuleti angu-384, 96, 24, 12, 6-wemithombo.

■ I-DNA Eraser ingasusa ngokushesha ama-genome akhululiwe, inciphise kakhulu umthelela emiphumeleni yokuhlola elandelayo.

■ Isistimu ye-RT ethuthukisiwe kanye ne-qPCR yenza ukuloba okuhlanekezela okuzinyathelo ezimbili kwe-RT-PCR kusebenze kahle futhi i-PCR icace kakhulu, futhi imelane kakhulu ne-RT-qPCR reaction inhibitors.

Isicelo sekhithi

Ububanzi bokusebenza: amaseli akhulisiwe.

- I-RNA ekhishwe ngesampula ye-lysis: isebenza kuphela kusifanekiso se-RT-qPCR sale kit.

- Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlaziywa kwesisho sofuzo, ukuqinisekiswa komphumela wokuthulisa ufuzo we-siRNA-mediated, ukuhlolwa kwezidakamizwa, njll.

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-4℃;Ingxenye II kufanele igcinwe ku -20 ℃.

I-Foregene Protease Plus II kufanele igcinwe ku-4℃, ingafrizi ku -20℃.

I-Reagent 2×Direct qPCR Mix-SYBR kufanele igcinwe ku -20℃ ebumnyameni;uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).Sike saba ngumkhiqizi onesipiliyoni.Ukuzuza iningi lezitifiketi zakho ezibalulekile zemakethe yayo ye-Super Purchasing for China Q160/Q320 Real-Time PCR, Uma kufanele ube nentshisekelo cishe kunoma yiziphi izimpahla, khumbula ukuzizwa ukhululekile ngempela ukuxhumana nathi ukuze uthole amanye amaqiniso noma uqiniseke ukuthi usithumelela i-imeyili ngokufanele, sizokuphendula emahoreni angama-24 kanye nekhotheshini enhle kakhulu izonikezwa.
Super Purchasing forI-PCR yaseChina, I-PCR Yesikhathi Sangempela, Ngomoya othakazelisayo "wokusebenza kahle kakhulu, ukunethezeka, ukusebenziseka nokusungula izinto ezintsha", futhi ngokuhambisana nesiqondiso esinjalo "sekhwalithi enhle kodwa amanani angcono," kanye "nesikweletu somhlaba wonke", silwela ukubambisana nezinkampani zezingxenye zezimoto emhlabeni wonke ukuze senze ubambiswano oluwinayo.