Kungaba indlela enhle yokuthuthukisa izixazululo nesevisi yethu.Umgomo wethu kuzoba ukwakha imikhiqizo eqanjiwe kubathengi abanolwazi oluphezulu lokusebenza lwe-Good Quality Plasmid DNA Mini Purification Kit, Ngokuvamile sibheka ubuchwepheshe kanye nabathengi njengabahamba phambili.Ngokuvamile senza umsebenzi wenziwe kanzima ukuze sidale amanani amahle kubathengi bethu futhi sinikeze abathengi bethu okuthengiswayo namasevisi angcono kakhulu.
Kungaba indlela enhle yokuthuthukisa izixazululo nesevisi yethu.Umgomo wethu kuzoba ukwakha imikhiqizo eqanjiwe kubathengi abanolwazi olusezingeni eliphezulu lokusebenzaUkuhlanzwa kwe-nucleic acid yaseChina kanye nePlasmid DNA Purification, Sigxila ekuhlinzekeni isevisi kumakhasimende ethu njengento ebalulekile ekuqiniseni ubudlelwano bethu besikhathi eside.Ukutholakala kwethu okuqhubekayo kokuthengiswayo kwebanga eliphezulu kuhlanganiswe nesevisi yethu enhle kakhulu yangaphambi kokuthengisa nangemuva kokuthengisa kuqinisekisa ukuncintisana okuqinile emakethe eya ngokuya yaba yimbulunga yonke.Sizimisele ukubambisana nabangane bebhizinisi abavela ekhaya nabaphesheya futhi sakhe ikusasa elihle ndawonye.

Izincazelo zekhithi

Imikhiqizo yochungechunge lwe-FOREGENE'S nucleic acid isizinda kanye nokuhlanzwa ingakhishwa futhi ihlanzwe kuma-nucleic acid ekhwalithi ephezulu kusuka emithonjeni ehlukahlukene kanye namasampula ezingxenye eziyinkimbinkimbi.Imikhiqizo yochungechunge lokukhishwa kwe-DNA isekelwe kumakholomu okuhlanza ulwelwesi lwe-silica ye-DNA Kuphela kanye nama-reagents efomula ahlukile angahlanza i-DNA yekhwalithi ephezulu kusukela kumasampuli ahlukahlukene.

I-General Plasmid Mini Kit isebenzisa ubuchwepheshe bekholomu yokuhlanza ye-DNA-Only kanye nefomula ye-SDS lysis esebenzayo ukuze kutholwe i-plasmid DNA yekhwalithi ephezulu kubhaktheriya phakathi nemizuzu engu-20.I-DNA-Kuphela ulwelwesi lwejeli ye-silica esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-FOREGENE, engakhanga i-DNA kahle futhi ngokuqondile, futhi isuse i-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngezinga elikhulu kakhulu.Umthamo omkhulu wokubopha we-plasmid DNA yekholomu yokuhlanza ye-DNA Kuphela ngu-60μg we-DNA.Inani le-plasmid DNA ekhishwe empeleni lihlobene nomthamo wesisombululo sebhaktheriya, inani lamakhophi e-plasmid (ikhophi ephezulu, ikhophi ephansi), izinkinga, izimo zesiko kanye nezinye izici.

Imininingwane

Izici&izinzuzo

- Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-plasmid DNA ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ukungcoliswa kwendawo yokucwaninga nge-RNase yangaphandle.

- Isivinini esisheshayo: Ukukhishwa kwe-Plasmid DNA kungaqedwa kungakapheli imizuzu engama-20.

- Kuphephile: Akukho kukhishwa kwe-organic reagent edingekayo.

- Ikhwalithi ephezulu: I-plasmid etholwe ngokukhipha ayinayo i-RNA, ayikho i-RNase kanye nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ukukhishwa kwe-Plasmid DNA kanye nokuhlanzwa kwamabhaktheriya e-Gram-negative.

Ukugeleza komsebenzi

Umdwebo

Isitoreji nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2–8℃.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37℃ imizuzu engu-10 ukuze uncibilikise imvula, futhi uyixube ngaphambi kokuyisebenzisa.

Kungaba indlela enhle yokuthuthukisa izixazululo nesevisi yethu.Umgomo wethu kuzoba ukwakha imikhiqizo eqanjiwe kubathengi abanolwazi oluphezulu lokusebenza lwe-Good Quality Plasmid DNA Mini Purification Kit, Ngokuvamile sibheka ubuchwepheshe kanye nabathengi njengabahamba phambili.Ngokuvamile senza umsebenzi wenziwe kanzima ukuze sidale amanani amahle kubathengi bethu futhi sinikeze abathengi bethu okuthengiswayo namasevisi angcono kakhulu.
Ikhwalithi EnhleUkuhlanzwa kwe-nucleic acid yaseChina kanye nePlasmid DNA Purification, Sigxila ekuhlinzekeni isevisi kumakhasimende ethu njengento ebalulekile ekuqiniseni ubudlelwano bethu besikhathi eside.Ukutholakala kwethu okuqhubekayo kokuthengiswayo kwebanga eliphezulu kuhlanganiswe nesevisi yethu enhle kakhulu yangaphambi kokuthengisa nangemuva kokuthengisa kuqinisekisa ukuncintisana okuqinile emakethe eya ngokuya yaba yimbulunga yonke.Sizimisele ukubambisana nabangane bebhizinisi abavela ekhaya nabaphesheya futhi sakhe ikusasa elihle ndawonye.