Inhloso yethu njalo ukwanelisa amakhasimende ethu ngokunikeza ukwesekwa kwegolide, inani eliphakeme kanye nekhwalithi ephezulu Yomkhiqizi Oholayo we-China Viral Rna&DNA Extraction Kit ye-Spin Column Method, Sithemba ngobuqotho ukukunikeza wena nenkampani yakho isiqalo esihle.Uma kukhona esizokwenza ukufeza izidingo zakho, sizokwengeza kunokujabula ukwenza kanjalo.Siyakwamukela endaweni yethu yokukhiqiza ukuze udlule.
Inhloso yethu njalo ukwanelisa amakhasimende ethu ngokunikeza ukwesekwa kwegolide, inani eliphakeme kanye nekhwalithi ephezulu yeIkhithi yokukhipha i-Rna ne-DNA yaseChina, Ukukhishwa kwe-Nucleic Acid, Inhloso yethu ukusiza amakhasimende ukuthi abone izinhloso zawo.Besenza imizamo emikhulu yokufeza lesi simo esiwinayo futhi sikwamukela ngobuqotho ukuthi uhlanganyele nathi.Ngamafuphi, uma usikhetha, ukhetha ukuphila okuphelele.Siyakwamukela ukuvakashela imboni yethu futhi wamukele i-oda lakho!Ukuze uthole eminye imibuzo, akufanele ungangabazi ukusithinta.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (ongakukhetha).Inhloso yethu ngaso sonke isikhathi ukwanelisa amakhasimende ethu ngokunikeza ukusekelwa kwegolide, inani eliphakeme kanye nekhwalithi ephezulu Yomkhiqizi Oholayo we-China Viral Rna&DNA Extraction Kit of Spin Column Method, Siqala ngethemba elihle kakhulu inkampani yakho yokukunikeza indlela enhle yokukunikeza.Uma kukhona esizokwenza ukufeza izidingo zakho, sizokwengeza kunokujabula ukwenza kanjalo.Siyakwamukela endaweni yethu yokukhiqiza ukuze udlule.
Umkhiqizi Ohamba phambili we-China Rna&DNA Extraction Kit,Ukukhishwa kwe-Nucleic Acid, Inhloso yethu ukusiza amakhasimende ukuthi abone izinhloso zawo.Besenza imizamo emikhulu yokufeza lesi simo esiwinayo futhi sikwamukela ngobuqotho ukuthi uhlanganyele nathi.Ngamafuphi, uma usikhetha, ukhetha ukuphila okuphelele.Siyakwamukela ukuvakashela imboni yethu futhi wamukele i-oda lakho!Ukuze uthole eminye imibuzo, akufanele ungangabazi ukusithinta.