Ngeke nje sizame konke okusemandleni ethu ukukunikeza imikhiqizo nezinsizakalo ezivelele kuye wonke umthengi oyedwa, kodwa futhi sikulungele ukuthola noma isiphi isiphakamiso esinikezwa abathengi bethu Ngenani Elikhethekile le-Magpure Ffpe Rna Kit ye-Nucleic Acid Isolation & Purification Kit, Ukuhlinzeka ngamathemba ngemishini emikhulu nezisombululo, futhi njalo ukuthuthukisa umshini omusha kuyizinjongo zebhizinisi lenkampani yethu.Sibheka phambili ngokubambisana kwakho.
Ngeke nje sizame konke okusemandleni ethu ukukunikeza imikhiqizo evelele namasevisi kumthengi ngamunye, kodwa futhi sikulungele ukuthola noma isiphi isiphakamiso esinikezwa abathengi bethuI-China Rna/DNA Extraction Purification kanye ne-Nucleic Acid Isolation, Siphikelela esimisweni esithi “Isikweletu sibe esiyinhloko, Amakhasimende eyinkosi futhi Ikhwalithi ibe engcono kakhulu”, besilokhu sibheke ngabomvu ukubambisana nabo bonke abangane ekhaya nabaphesheya futhi sizokwakha ikusasa eliqhakazile lebhizinisi.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa i-spin ikholomu kanye nefomulaithuthukiswe inkampani yethu, engakwazi ukukhipha i-RNA ehlanzekile nesezingeni eliphezulu ezicutshini zezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ephumelelayo ye-DNA-Cleaning, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic kusuka ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi

Ulwazi lomkhiqizo

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

Izihloko ezicashuniwe

1.IF:18.808:Zheng, Q., Qin, F., Luo, R., et al.I-mRNA-Layishwa I-Lipid-Like Nanoparticles Yokuhlelwa Kwesisekelo Sesibindi Ngokuthuthukiswa Komklamo Oyinhlanganisela Emaphakathi.Adv.Umsebenzi.UMater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:He X, Hong W, Yang J, et al.I-apoptosis ezenzakalelayo yamaseli ekulungiseleleni i-stem cell therapy isebenzisa imiphumela yokuzivikela ngokukhululwa kwe-phosphatidylserine.I-Signal Transduct Target Ther.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.IF:17.97:Dai Z, Liu H, Liao J, et al.Ukuguqulwa kwe-N7-Methylguanosine tRNA kuthuthukisa ukuhumusha kwe-oncogenic mRNA futhi kukhuthaze ukuqhubeka kwe-intrahepatic cholangiocarcinoma.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.IF:9.225:Cao X, Shu Y, Chen Y, et al.Ukuguqulwa kwe-M6A kwe-Mettl14-Mediated Kusiza Ukuvuselelwa Kwesibindi Ngokugcina I-Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

I-RNA isoaltion kits ye eminye imithombo yesampulaayatholakala:

Iseli, isitshalo, igciwane, igazi, njll.Ngeke nje sizame okukhulu kakhulu ukukunikeza imikhiqizo evelele kanye nezinsizakalo kumthengi ngamunye, kodwa futhi sikulungele ukwamukela noma isiphi isiphakamiso esinikezwa abathengi bethu Ngenani Elikhethekile le-Magpure Ffpe Rna Kit ye-Nucleic Acid Isolation & Purification Kit, Ukunikeza amathemba ngemishini emikhulu nezisombululo, futhi ukuhlale kusungula izinhloso zebhizinisi ezintsha zenkampani yethu.Sibheka phambili ngokubambisana kwakho.
Intengo Ekhethekile yeI-China Rna/DNA Extraction Purification kanye ne-Nucleic Acid Isolation, Siphikelela esimisweni esithi “Isikweletu sibe esiyinhloko, Amakhasimende eyinkosi futhi Ikhwalithi ibe engcono kakhulu”, besilokhu sibheke ngabomvu ukubambisana nabo bonke abangane ekhaya nabaphesheya futhi sizokwakha ikusasa eliqhakazile lebhizinisi.

I-RNA ayikhishiwe noma isivuno se-RNA siphansi

Kuvame ukuba nezinto ezihlukahlukene ezithinta ukusebenza kahle kokululama, njengalezi: okuqukethwe kwesampula yezicubu ze-RNA, indlela yokusebenza, ivolumu ye-elution, njll.

1. Ukugeza kweqhwa noma i-cryogenic (4 °C) i-centrifugation yenziwa ngesikhathi sokusebenza.

Okutuswayo: Sebenzisa ekamelweni lokushisa (15-25 ° C) kuyo yonke inqubo, ungabhavi eqhweni kanye ne-centrifuge emazingeni okushisa aphansi.

2. Ukulondolozwa kwesampula okungalungile noma isikhathi sokugcina isampula eseqile.

Okutuswayo: Gcina amasampula ku--80 °C noma ufrize ku-nitrogen ewuketshezi futhi ugweme ukusetshenziswa okuphindaphindiwe kokuqhwaza;zama ukusebenzisa izicubu ezintsha noma amaseli akhulisiwe ukuze kukhishwe i-RNA.

3. Isampula yesampula enganele.

Okutuswayo: Uma wenza izicubu zibe homogenizing, qinisekisa ukuthi izicubu zihlanganiswe ngendlela eyanele nokuthi amaseli ezicubu ahlukaniswe ngokwanele ukuchaza ukukhululwa kwe-RNA.

4. I-eluent ayengezwanga ngendlela efanele.

Okunconyiwe: Qinisekisa ukuthi i-RNase-Free ddH₂U-O wengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza.

5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RL2 noma ku-Buffer RW2.

Isincomo: Landela imiyalelo, engeza ivolumu efanele ye-ethanol ephelele ku-Buffer RL2 kanye ne-Buffer RW2 bese uxuba kahle ngaphambi kokusebenzisa ikhithi.

6. Umthamo wesampula wezicubu awufanelekile.

Isincomo: Sebenzisa i-10-20 mg yezicubu noma (1-5) × 10⁶amaseli nge-500 μl buffer RL1, njengoba ukusetshenziswa kwezicubu ngokweqile kungaholela ekukhishweni kwe-RNA okuncishisiwe.

7. Ivolumu ye-elution engafanele noma ukukhishwa okungaphelele.

Okutuswayo: Ivolumu ye-elution yekholomu yokuhlanza ingu-50-200 μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi kunwetshwe isikhathi sokubeka izinga lokushisa legumbi ngemva kokwengeza i-ddH eshisiwe ye-RNase-Free₂O, isb imizuzu emi-5-10.

8.Ikholomu yokuhlanza inensalela ye-ethanol ngemva kokugeza kwe-Buffer RW2.

Okutuswayo: Uma kunensalela ye-ethanol ngemva kokugeza kwe-Buffer RW2, i-tube centrifugation engenalutho ye-1min, isikhathi sokusebenza kwe-tube centrifugation esingenalutho singanyuswa sibe yi-2min, noma ikholomu yokuhlanza ingabekwa ekamelweni lokushisa imizuzu emi-5 ukuze kukhishwe ngokwanele i-ethanol esele.

I-RNA ehlanziwe yehlisiwe

Ikhwalithi ye-RNA ehlanzekile ihlobene nezici ezifana nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, nokukhohlisa, njll.

1. Amasampula ezicubu awagcinwa ngesikhathi.

Okutuswayo: Uma amasampula ezicubu noma amaseli engasetshenziswa ngesikhathi esifanele ngemva kokuqoqwa, londoloza ngokushesha i-cryopreserve ku- -80 °C noma i-nitrogen ewuketshezi.Ukuze ukhiphe i-RNA, sebenzisa ithishu esanda kuthathwa noma isampula yeseli noma nini lapho kunokwenzeka.

2. Ukuqhwaza okuphindaphindiwe kwamasampula ezicubu.

Okutuswayo: Uma ugcina amasampula ezicubu, kuhle kakhulu ukuwasika abe izingcezu ezincane ukuze alondolozwe, bese ususa ingxenye eyodwa yezingcezu lapho uzisebenzisa ukugwema ukuncibilika okubandayo kwesampula kanye nokonakala kwe-RNA.

3. I-RNase yethulwa noma ingagqoki amagilavu ​​alahlwayo, imaski, njll. phakathi nokuhlinzwa.

Okunconyiwe: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu emagumbini okukhohlisa e-RNA ahlukene futhi ithebula liyasulwa ngaphambi kokuhlolwa.

Gqoka amagilavu ​​alahlwayo kanye nemaski ngesikhathi sokuhlolwa ukuze unciphise ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase.

4. Ama-reagents angcoliswe yi-RNase ngesikhathi sokusetshenziswa.

Okunconyiwe: Faka esikhundleni Ikhithi Yokuhlukanisa Yezilwane Ephelele Yenani Le-RNA ukuze uthole ukuhlolwa okuhlobene.

5. Amashubhu e-centrifuge, amathiphu, njll. asetshenziswa ekukhohlisweni kwe-RNA angcoliswe yi-RNase.

Okutuswayo: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu, amapayipi, njll. asetshenziswa ekukhishweni kwe-RNA konke Akuna-RNAse.

I-RNA etholiwe ehlanziwe ithinta ukuhlola okwehla nomfula

I-RNA ehlanjululwe ngekholomu yokuhlanza, uma ama-ion kasawoti, okuqukethwe kwamaprotheni kukukhulu kakhulu kuzothinta isilingo esisezansi nomfula, njengokuthi: ukuloba okuhlanekezelwe,Northern Blot et al.

1. I-RNA ekhishiwe inezinsalela ze-ion kasawoti.

Okunconyiwe: Qinisekisa ukuthi ivolumu elungile ye-ethanol yengezwe ku-Buffer RW2 futhi wenze amakholomu okuhlanza ama-2 ageza ngesivinini esimaphakathi esikhonjiswe ukusebenza;uma kukhona noma iyiphi insalela ye-ion kasawoti, shiya ikholomu yokuhlanza ku-Buffer RW2 imizuzu engu-5 ekamelweni lokushisa futhi wenze i-centrifugation ukwandisa ukukhishwa kokungcoliswa kukasawoti.

2. Izinsalela ze-Ethanol ku-RNA elutioned.

Isincomo: Qinisekisa ukuthi ngemva kokugeza kwe-buffer RW2, yenza i-tube centrifugation operation engenalutho ngesivinini se-centrifugation esiboniswe ukusebenza, ukwandisa isikhathi sokusebenza kwe-tube centrifugation engenalutho kumaminithi angu-2 uma kusekhona insalela ye-ethanol, noma uyishiye ekamelweni lokushisa imizuzu engu-5 ngemva kwe-tube centrifugation engenalutho ukuze ukhulise ukukhishwa kwe-thanol.