Ngokunamathela enkolelweni ethi "Ukudala izimpahla ezisezingeni eliphezulu nokudala abangani nabantu abavela yonke indawo emhlabeni", sivame ukubeka ukuthakazelisa kwabathengi kuqala ngentengo enengqondo I-China Genomic Nucleic Acid Purification Rna Isolation DNA Extraction Reagent Kit, Ikhwalithi enhle enhle, izindleko ezincintisanayo, sazise ngokushesha ukulethwa kwesigaba sakho futhi kudinga ukunikezwa okuthembekile kwesigaba ngasinye. angakwazisa kalula ngokufanele.
Ngokunamathela enkolelweni “yokudala izimpahla ezisezingeni eliphezulu nokudala abangani nabantu abavela yonke indawo emhlabeni”, sivame ukubeka ukuthakaseleka kwabathengi kuqalaI-China Blood Dna, Ikhithi yokukhipha, Sikholelwa ukuthi ngesevisi yethu ehlala njalo enhle kakhulu ungathola ukusebenza okungcono kakhulu kanye nezixazululo ezingabizi kakhulu kithi isikhathi eside .Sizibophezela ekuhlinzekeni ngezinsizakalo ezingcono futhi sidale inani elithe xaxa kuwo wonke amakhasimende ethu.Ngethemba ukuthi singakha ikusasa elingcono ndawonye.

Imininingwane

50 Preps, 100 Preps, 250 Preps

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu ye-buffer eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingu-12 ekamelweni lokushisa (15–25 ℃) kanye no-2–8 ℃ isikhathi eside. Ngokunamathela enkolelweni “Yokudala izimpahla ezisezingeni eliphezulu nokwakha ubungane nabantu abavela yonke indawo emhlabeni”, ngokuvamile sibeka ukuthakaseleka kwabathengi kwasekuqaleni ngenani elizwakalayo I-China Genomic Purification DNA, I-complication ye-China Genomic Purification izindleko zokuthenga, ukulethwa okusheshayo kanye nomhlinzeki onokwethenjelwa kuqinisekisiwe Sicela usazise imfuneko yakho yobuningi ngaphansi kwesigaba sosayizi ngamunye ukuze sikwazi ukukwazisa kalula ngokufanele.
Intengo enengqondo i-China Blood DNA,Ikhithi yokukhipha, Sikholelwa ukuthi ngesevisi yethu ehlala njalo enhle kakhulu ungathola ukusebenza okungcono kakhulu kanye nezixazululo ezingabizi kakhulu kithi isikhathi eside .Sizibophezela ekuhlinzekeni ngezinsizakalo ezingcono futhi sidale inani elithe xaxa kuwo wonke amakhasimende ethu.Ngethemba ukuthi singakha ikusasa elingcono ndawonye.