Ukunamathela enkolelweni yakho "Yokudala izixazululo zekhwalithi ephezulu kanye nokukhiqiza abangani nabantu abavela emhlabeni wonke", sihlala sibeka ukuthakazelisa kwamakhasimende ukuthi kuqale ngakho Ukulethwa Okusheshayo kwe-China Rna Isolation DNA Extraction Reagent Kit, Ngamafuphi, uma usikhetha, ukhetha ukuba khona okufanele.Uyemukelwa ukuvakashela imboni yethu futhi wamukele ukuthola kwakho!Ngemibuzo eyengeziwe, khumbula ukuthi ngokuvamile ungangabazi ukuxhumana nathi.
Ukunamathela enkolelweni yakho "Yokudala izixazululo zekhwalithi ephezulu nokukhiqiza abangani nabantu abavela kuwo wonke umhlaba", sihlala sibeka umdlandla wamakhasimende ukuze siqale ngawoIkhithi yokukhipha iDNA yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Sigxila ekuhlinzekeni isevisi kumakhasimende ethu njengento ebalulekile ekuqiniseni ubudlelwano bethu besikhathi eside.Ukutholakala kwethu okuqhubekayo kwemikhiqizo yezinga eliphezulu kuhlanganiswe nesevisi yethu enhle kakhulu yokuthengisa ngaphambi kokuthengisa nangemva kokuthengisa kuqinisekisa ukuncintisana okuqinile emakethe eya ngokuya yanda umhlaba wonke.Besizimisele ukubambisana nabangane bebhizinisi abavela ekhaya naphesheya futhi sakhe ikusasa elihle ndawonye.

Imininingwane

50 Preps, 100 Preps, 250 Preps

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu ye-buffer eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingu-12 ekamelweni lokushisa (15–25 ℃) kanye no-2–8 ℃ isikhathi eside. Ngokunamathela enkolelweni yakho “Yokudala izixazululo zekhwalithi ephezulu nokukhiqiza abangani nabantu abavela kuwo wonke umhlaba”, sihlala sibeka ukuthakazelisa kwamakhasimende ukuqalisa ngakho Ukulethwa Okusheshayo kwe-China Genomic Nuclear, I-Acsoid Nuclear DNA you , ukhetha ukuba khona okufanele.Uyemukelwa ukuvakashela imboni yethu futhi wamukele ukuthola kwakho!Ngemibuzo eyengeziwe, khumbula ukuthi ngokuvamile ungangabazi ukuxhumana nathi.
Ukulethwa Okusheshayo kweIkhithi yokukhipha iDNA yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Sigxila ekuhlinzekeni isevisi kumakhasimende ethu njengento ebalulekile ekuqiniseni ubudlelwano bethu besikhathi eside.Ukutholakala kwethu okuqhubekayo kwemikhiqizo yezinga eliphezulu kuhlanganiswe nesevisi yethu enhle kakhulu yokuthengisa ngaphambi kokuthengisa nangemva kokuthengisa kuqinisekisa ukuncintisana okuqinile emakethe eya ngokuya yanda umhlaba wonke.Besizimisele ukubambisana nabangane bebhizinisi abavela ekhaya naphesheya futhi sakhe ikusasa elihle ndawonye.